caa a22 4500 475832310 CHVBK 20180406123843.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s100960050475 doi (NATIONALLICENCE)springer-10.1007/s100960050475 Evaluation of Phenotypic and Genotypic Markers for Characterisation of the Emerging Gastroenteritis Pathogen Salmonella hadar [Elektronische Daten] [S. Valdezate, A. Echeita, R. Díez, M. A. Usera]  Over the last 4 years Salmonella hadar has increasingly been isolated in Europe in conjunction with food-borne gastroenteritis. The aim of this study was to evaluate epidemiological methods (phage typing, antimicrobial susceptibility testing, DNA plasmid analysis, ribotyping and pulsed-field gel electrophoresis analysis) for characterising Salmonella hadar isolates. The 100% phage typeability of isolates and the high discriminatory index of 0.8856 suggest that phage typing is the method of choice. In order to obtain subdivisions of the most frequent Salmonella hadar phage types, a combination of molecular methods, such as ribotyping performed with BglI and EcoRI or pulsed-field gel electrophoresis using XbaI and XhoI, would be desirable as the usefulness of each technique varies with the phage type being analysed. Of note was the high (86%) rate of resistance to tetracycline and nalidixic acid but full susceptibility to ciprofloxacin in the strains studied. Springer-Verlag Berlin Heidelberg, 2000 Valdezate S. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut Echeita A. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut Díez R. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut Usera M. A. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut https://doi.org/10.1007/s100960050475 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050475 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valdezate S. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut NATIONALLICENCE 700 1- Echeita A. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut NATIONALLICENCE 700 1- Díez R. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut NATIONALLICENCE 700 1- Usera M. A. Laboratorio Nacional de Referencia de Salmonella y Shigella de España (LNRSSE), Centro Nacional de Microbiología, Instituto Carlos III, Mahadajonda, Madrid 28220, Spain e-mail: mausera@isciii.es, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer