caa a22 4500 475832418 CHVBK 20180406123843.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s100960000324 doi (NATIONALLICENCE)springer-10.1007/s100960000324 Isolation of Kingella kingae from Synovial Fluids Using Four Commercial Blood Culture Bottles [Elektronische Daten] [B. Høst, H. Schumacher, J. Prag, M. Arpi]  According to the literature, Kingella kingae may be an underdiagnosed cause of joint and bone infections in children. The use of the Bactec blood culture system for culture of joint fluids has dramatically improved the isolation of this fastidious bacterium. The aim of this study was to test the recovery rate and detection time of four commercial blood culture systems: three different BacT/Alert (Organon Teknika, USA) bottles and one Bactec (Becton Dickinson Microbiology Systems, USA) bottle, all inoculated with Kingella kingae strains mixed with pooled synovial fluids. For each strain the same inoculum and volume of synovial fluid was distributed into each of the four bottles. All 24 strains tested grew in the BacT/Alert Aerobic (100%) and the BacT/Alert Pedi-BacT (100%) bottles. Twenty-one strains grew in the BacT/Alert FAN aerobic (88%) bottle, and 15 strains grew in the Bactec Plus Aerobic F (63%) bottle, in both systems within 12 days (P<0.01). The Kingella kingae strains were first detected in the BacT/Alert Pedi-BacT bottles (P<0.001). The results were reproducible. The BacT/Alert blood culture bottles were superior to previously described blood culture systems in isolating Kingella kingae from synovial fluid, even with small inoculums and small volumes of synovial fluid. Springer-Verlag Berlin Heidelberg, 2000 Høst B. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut Schumacher H. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut Prag J. Department of Clinical Microbiology, Viborg Hospital, 8800 Viborg, Denmark, DK aut Arpi M. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut https://doi.org/10.1007/s100960000324 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000324 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Høst B. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut NATIONALLICENCE 700 1- Schumacher H. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut NATIONALLICENCE 700 1- Prag J. Department of Clinical Microbiology, Viborg Hospital, 8800 Viborg, Denmark, DK aut NATIONALLICENCE 700 1- Arpi M. Department of Clinical Microbiology, Aarhus University Hospital, Nørrebrogade 44, 8000 Aarhus C, Denmark e-mail: akh.grp02s.mrp@aaa.dk, DK aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer