caa a22 4500 475832450 CHVBK 20180406123844.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s100960000321 doi (NATIONALLICENCE)springer-10.1007/s100960000321 Molecular Typing of Bacteria Directly from Cerebrospinal Fluid [Elektronische Daten] [M. C. Enright, K. Knox, D. Griffiths, D. W. M. Crook, B. G. Spratt]  Using Streptococcus pneumoniae as an example, the ability of multilocus sequence typing (MLST) to characterise isolates directly from cerebrospinal fluid (CSF) was investigated. A nested multiplex polymerase chain reaction method that amplifies the seven housekeeping gene fragments used for pneumococcal MLST was applied to 30 CSF samples from suspected cases of bacterial meningitis. The fragments were amplified from all 14 samples from which Streptococcus pneumoniae was cultured, and, after direct sequencing, the allelic profiles obtained from ten of the samples corresponded to those of clones previously associated with invasive pneumococcal disease. MLST could also predict the penicillin susceptibility and serotype of the CSF isolates. Springer-Verlag Berlin Heidelberg, 2000 Enright M. C. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut Knox K. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut Griffiths D. Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK, GB aut Crook D. W. M. Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK, GB aut Spratt B. G. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut https://doi.org/10.1007/s100960000321 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000321 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Enright M. C. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut NATIONALLICENCE 700 1- Knox K. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut NATIONALLICENCE 700 1- Griffiths D. Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK, GB aut NATIONALLICENCE 700 1- Crook D. W. M. Department of Microbiology, John Radcliffe Hospital, Oxford University, Oxford OX3 9DU, UK, GB aut NATIONALLICENCE 700 1- Spratt B. G. Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, South Parks Road, University of Oxford, Oxford OX1 3FY, UK e-mail: mark.enright@ceid.ox.ac.uk, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer