caa a22 4500 475832515 CHVBK 20180406123844.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s100960000322 doi (NATIONALLICENCE)springer-10.1007/s100960000322 Detection of Isospora belli by Polymerase Chain Reaction Using Primers Based on Small-Subunit Ribosomal RNA Sequences [Elektronische Daten] [A. Müller, R. Bialek, G. Fätkenheuer, B. Salzberger, V. Diehl, C. Franzen]  The aim of the present study was to use small-subunit (SSU)-rRNA sequences of Isospora belli to design specific primer pairs and a hybridization probe for the detection of Isospora belli in human samples by PCR and Southern blot hybridization. PCR amplification with the primer pairs produced correct DNA fragments with target DNA from samples of Isospora belli-infected patients and from cloned SSU-rRNA of Isospora belli. The nature of the PCR products was confirmed by Southern blot hybridization. No amplification was seen with template DNA extracted from other parasites. Although Isospora belli infections can be easily diagnosed using light microscopy, molecular-based techniques may prove useful as an additional diagnostic tool. Springer-Verlag Berlin Heidelberg, 2000 Müller A. Department of Pediatrics, University of Cologne, Cologne, Germany, DE aut Bialek R. Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany, DE aut Fätkenheuer G. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut Salzberger B. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut Diehl V. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut Franzen C. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut https://doi.org/10.1007/s100960000322 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000322 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Müller A. Department of Pediatrics, University of Cologne, Cologne, Germany, DE aut NATIONALLICENCE 700 1- Bialek R. Institute for Tropical Medicine, University of Tübingen, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Fätkenheuer G. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut NATIONALLICENCE 700 1- Salzberger B. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut NATIONALLICENCE 700 1- Diehl V. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut NATIONALLICENCE 700 1- Franzen C. Department of Internal Medicine I, University of Cologne, Joseph-Stelzmann Strasse 9, 50924 Cologne, Germany e-mail: Caspar.Franzen@Uni-Koeln.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer