caa a22 4500 475832671 CHVBK 20180406123844.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s100960050494 doi (NATIONALLICENCE)springer-10.1007/s100960050494 Differentiation of Entamoeba histolytica and Entamoeba dispar Cysts Using Polymerase Chain Reaction on DNA Isolated from Faeces with Spin Columns [Elektronische Daten] [J. J. Verweij, J. Blotkamp, E. A. T. Brienen, A. Aguirre, A. M. Polderman]  Since Entamoeba histolytica and Entamoeba dispar were formally recognized as two different species at the World Health Organization (WHO)/Pan American Health Organization (PAHO)/United Nations Educational, Scientific and Cultural Organization (UNESCO) meeting in Mexico City in 1997, the specific differentiation of the two morphologically identical species would seem relevant in clinical diagnosis. Several polymerase chain reaction (PCR)-based methods have been described and used successfully, but methods for DNA isolation from cysts in stool samples are time-consuming and problematic due to inhibitory factors in faeces. The use of the slightly modified QIAamp tissue method (Qiagen, Germany) for DNA isolation was evaluated in 657 unpreserved faecal samples from cases of suspected Entamoeba histolytica/Entamoeba dispar infection. In only 1.7% of the cases was PCR hampered by inhibitors present in the faeces. The DNA isolation procedure was found to be rapid, simple and one that could easily be implemented in a routine diagnostic setting. In 98.8% of Entamoeba histolytica/Entamoeba dispar cyst-positive faecal samples, the true identity of the cysts could be determined using PCR specific for Entamoeba histolytica and Entamoeba dispar, respectively. Springer-Verlag Berlin Heidelberg, 2000 Verweij J. J. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut Blotkamp J. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut Brienen E. A. T. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut Aguirre A. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK, GB aut Polderman A. M. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut https://doi.org/10.1007/s100960050494 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050494 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verweij J. J. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut NATIONALLICENCE 700 1- Blotkamp J. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut NATIONALLICENCE 700 1- Brienen E. A. T. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut NATIONALLICENCE 700 1- Aguirre A. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK, GB aut NATIONALLICENCE 700 1- Polderman A. M. Department of Parasitology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands e-mail: j.j.verweij@lumc.nl, NL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer