caa a22 4500 475832752 CHVBK 20180406123844.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s100960050442 doi (NATIONALLICENCE)springer-10.1007/s100960050442 Survival and Growth of Burkholderia cepacia Within the Free-Living Amoeba Acanthamoeba polyphaga [Elektronische Daten] [P. Landers, K. G. Kerr, T. J. Rowbotham, J. L. Tipper, P. M. Keig, E. Ingham, M. Denton]  The aim of this study was to investigate whether Burkholderia cepacia is capable of survival and growth within the free-living amoeba Acanthamoeba polyphaga using a differential immunofluorescence assay of bacterial-amoebal cocultures and viable counts of bacteria determined after amoebal lysis. The numbers of intra-amoebal bacteria and the numbers of infected amoebae increased over time; although, when heat-killed bacteria were used, no intracellular bacteria were observed. These findings should be taken into account in future studies of environmental reservoirs of Burkholderia cepacia. Springer-Verlag Berlin Heidelberg, 2000 Landers P. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut Kerr K. G. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut Rowbotham T. J. Leeds Public Health Laboratory, Leeds LS15 7TR, UK, GB aut Tipper J. L. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut Keig P. M. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut Ingham E. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut Denton M. Department of Microbiology, The General Infirmary at Leeds, Leeds LS1 3EX, UK, GB aut https://doi.org/10.1007/s100960050442 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050442 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Landers P. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut NATIONALLICENCE 700 1- Kerr K. G. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut NATIONALLICENCE 700 1- Rowbotham T. J. Leeds Public Health Laboratory, Leeds LS15 7TR, UK, GB aut NATIONALLICENCE 700 1- Tipper J. L. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut NATIONALLICENCE 700 1- Keig P. M. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut NATIONALLICENCE 700 1- Ingham E. Department of Microbiology, University of Leeds, Leeds LS2 9JT, UK e-mail: mickgk@leeds.ac.uk, GB aut NATIONALLICENCE 700 1- Denton M. Department of Microbiology, The General Infirmary at Leeds, Leeds LS1 3EX, UK, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer