caa a22 4500 475832787 CHVBK 20180406123845.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s100960050447 doi (NATIONALLICENCE)springer-10.1007/s100960050447 Evaluation of a Fluorescence Hybridisation Assay Using Peptide Nucleic Acid Probes for Identification and Differentiation of Tuberculous and Non-Tuberculous Mycobacteria in Liquid Cultures [Elektronische Daten] [E. Padilla, J. M. Manterola, O. F. Rasmussen, J. Lonca, J. Domínguez, L. Matas, A. Hernández, V. Ausina]  The performance was evaluated of a fluorescence in situ hybridisation assay using peptide nucleic acid probes (Dako Probe MTB Culture Confirmation Test; Dako, Denmark) for identification of Mycobacterium tuberculosis complex (MTC) organisms and differentiation between tuberculous and non-tuberculous mycobacteria (NTM) in material taken directly from Bactec 12B (Becton Dickinson, USA) and MB/BacT (Organon Teknika, USA) bottles. The test was applied to 129 smear-positive (Ziehl-Neelsen stain) clinical specimens, 48 previously identified clinical strains of mycobacteria (12 MTC and 36 NTM), and 51 reference strains (7 MTC and 44 NTM) which were all previously inoculated into Bactec 12B and MB/BacT bottles. The sensitivity and specificity of the assay for MTC-positive cultures was 87.6% and 100%, respectively, for Bactec 12B, and 100%, respectively, for MB/BacT. The sensitivity and specificity of the assay for NTM-positive cultures was 100% for both media. Springer-Verlag Berlin Heidelberg, 2000 Padilla E. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Manterola J. M. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Rasmussen O. F. Dako A/S, Department of Microbiology, Produktionsvej 42, 2600 Glostrup, Denmark, DK aut Lonca J. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Domínguez J. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Matas L. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Hernández A. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Ausina V. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut https://doi.org/10.1007/s100960050447 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050447 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Padilla E. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Manterola J. M. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Rasmussen O. F. Dako A/S, Department of Microbiology, Produktionsvej 42, 2600 Glostrup, Denmark, DK aut NATIONALLICENCE 700 1- Lonca J. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Domínguez J. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Matas L. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Hernández A. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut NATIONALLICENCE 700 1- Ausina V. Servicio de Microbiología, Hospital Universitario Germans Trias i Pujol, Carretera del Canyet s/n, 08916 Badalona, Barcelona, Spain, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer