caa a22 4500 475832876 CHVBK 20180406123845.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s100960050444 doi (NATIONALLICENCE)springer-10.1007/s100960050444 Comparison of Three Methods for Identification of Streptococcus milleri Group Isolates to Species Level [Elektronische Daten] [A. Limia, T. Alarcón, M. L. Jiménez, M. López-Brea]  A collection of 180 clinical isolates of Streptococcus milleri group were identified to species level using two phenotypic methods (a commercial system and the reference method based on differential phenotypic reactions) and a genotypic method (hybridisation of the 16S rRNA gene with species-specific probes) in order to evaluate the performance of the respective methods. A high level of agreement (80%) was observed between the results of the reference method and the genotypic method. The highest level of agreement was found for the species Streptococcus anginosus (83%), a high level of agreement (76%) also being achieved for Streptococcus constellatus and Streptococcus intermedius. The sensitivity of the commercial system compared to the genotypic method was 76% overall, but it was low (57.5%) for Streptococcus intermedius. Twenty-five strains belonged to the recently described CI strains. Springer-Verlag Berlin Heidelberg, 2000 Limia A. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut Alarcón T. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut Jiménez M. L. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut López-Brea M. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut https://doi.org/10.1007/s100960050444 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960050444 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Limia A. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut NATIONALLICENCE 700 1- Alarcón T. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut NATIONALLICENCE 700 1- Jiménez M. L. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut NATIONALLICENCE 700 1- López-Brea M. Department of Clinical Microbiology, Hospital Universitario de la Princesa, Diego de León 62, 28006 Madrid, Spain e-mail: mlbrea@microb.net, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer