caa a22 4500 475832922 CHVBK 20180406123845.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s100960000380 doi (NATIONALLICENCE)springer-10.1007/s100960000380 Improved Microbiological Techniques Using the Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis for Diagnosis and Follow-Up of Enterohaemorrhagic Escherichia coli Infection [Elektronische Daten] [C. Welinder-Olsson, E. Kjellin, M. Badenfors, B. Kaijser]  The aims of the present investigation were to evaluate the microbiological diagnostic procedures, especially polymerase chain reaction (PCR) versus culture and seroagglutination, in relation to the clinical features of enterohaemorrhagic Escherichia coli (EHEC) infection and to study the status of EHEC in the western part of Sweden. During 1997 and 1998, stool specimens from 3,948 patients were analysed by PCR for the presence of EHEC with verotoxin (VT)1- and/or VT2-producing DNA sequences. The stool specimens were also cultured for Escherichia coli O157 : H7, Salmonella, Campylobacter, Shigella and Yersinia. Fifty-five patients were positive by PCR. Thirty-nine patients were positive for EHEC by PCR and culture. Of these, 29 were infected with EHEC serogroup O157 : H7 strains. All EHEC isolates were analysed by pulsed-field gel electrophoresis (PFGE); 17 different clones were identified. Studies on the duration of the presence of EHEC in the gut showed that EHEC often disappears rather quickly, i.e. within 2 weeks. In one patient, however, EHEC remained for several months. In conclusion, PCR, rather than culture and agglutination, should be the method of choice for microbiological diagnosis of EHEC infection. PCR is more sensitive than culture for detecting EHEC in the gut. Springer-Verlag Berlin Heidelberg, 2000 Welinder-Olsson C. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut Kjellin E. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut Badenfors M. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut Kaijser B. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut https://doi.org/10.1007/s100960000380 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s100960000380 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Welinder-Olsson C. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut NATIONALLICENCE 700 1- Kjellin E. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut NATIONALLICENCE 700 1- Badenfors M. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut NATIONALLICENCE 700 1- Kaijser B. Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, 413 46 Göteborg, Sweden e-mail: bertil.kaijser@microbio.gu.se Tel.: +46-31-3244908 Fax: +46-31-410024, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer