caa a22 4500 475833708 CHVBK 20180406123848.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1007876703644 doi (NATIONALLICENCE)springer-10.1023/A:1007876703644 Effect of Vanadate and Insulin on Glucose Transport in Isolated Adult Rat Cardiomyocytes [Elektronische Daten] [Rajakumar Donthi, Barbara Huisamen, Amanda Lochner] It is now widely accepted that insulin stimulation of glucose uptake by muscle cells is due to the activation of protein kinase B, leading to the recruitment of glucose transporter proteins from an intracellular compartment to the plasma membrane. Vanadate is a protein tyrosine phosphatase (PTP) inhibitor and a known insulin mimetic agent. Vanadate causes an increase of glucose transport in various tissues, but the mechanism of stimulation is not clearly understood. Hence in the present study, we have compared the mechanism of 2-deoxy-D-glucose transport induced by vanadate and insulin in isolated rat cardiomyocytes. Vanadate stimulated deoxyglucose transport in a time- and concentration-dependent manner. Insulin (100 nM) and vanadate (5 mM) stimulated 2-deoxy-D-glucose transport on an average by 3- and 2-fold respectively over basal values. The stimulation of glucose transport was accompanied by an activation of protein kinase B (PKB). This study also revealed that the activation of PKB and stimulation of 2-deoxyglucose uptake by vanadate and insulin are inhibited by treatment with wortmannin, a specific inhibitor of phoshatidylinositol 3-kinase (PI 3-kinase). Hence, we conclude that both insulin and vanadate follow the same signalling pathway downstream of PI 3-kinase to stimulate 2-deoxy-D-glucose transport. Kluwer Academic Publishers, 2000 insulin nationallicence vanadate nationallicence wortmannin nationallicence cardiomyocytes nationallicence PI 3-kinase nationallicence PKB nationallicence 2-deoxy-D-glucose transport nationallicence Donthi Rajakumar Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut Huisamen Barbara Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut Lochner Amanda Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 14/5(2000-10-01), 463-470 0920-3206 14:5<463 2000 14 10557 https://doi.org/10.1023/A:1007876703644 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007876703644 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Donthi Rajakumar Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut NATIONALLICENCE 700 1- Huisamen Barbara Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut NATIONALLICENCE 700 1- Lochner Amanda Department of Medical Physiology and Biochemistry, Faculty of Medicine, University of Stellenbosch, 7505, Tygerberg, Republic of South Africa aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 14/5(2000-10-01), 463-470 0920-3206 14:5<463 2000 14 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer