caa a22 4500 475833716 CHVBK 20180406123848.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1023/A:1007893207279 doi (NATIONALLICENCE)springer-10.1023/A:1007893207279 Calcium Channel Blockers Reduce Blood Pressure in Part by Inhibiting Vascular Smooth Muscle Carbonic Anhydrase I [Elektronische Daten] [Ioan Puscas, Ludovic Gilau, Marcela Coltau, Radu Pasca, Gabriela Domuta, Michael Baican, Alex Hecht] Calcium channel blockers are a group of drugs used for the treatment of hypertension. Carbonic anhydrase (CA) I detected in vascular smooth muscle and in other cells in the organism has a major role in the acid-base balance and in vascular processes. Our previous work has proven that verapamil inhibits CA activity by a direct mechanism of action. Starting from our results in this article we studied in vitro and in vivo the effect of calcium channel blockers (verapamil and amlodipine) on erythrocyte CA I, on vascular smooth mucles CA I, and on arterial blood pressure values in human and in animals. Our in vitro and in vivo results have proved that verapamil and amlodipine are strong CA I inhibitors both in human erythrocytes and also in vascular smooth muscles in animals. In humans, calcium channel blockers studied here progressively reduce arterial blood pressure in hypertensive subjects, in parallel with progressive lowering of erythrocyte CA I activity in the normal range in normotensive subjects. From our point of view verapamil and amlodipine possess a dual mechanism of action: the first well-known action consists of their action on calcium channels. The second mechanism, suggested by us, directly acts on the vascular smooth muscle CA I isozyme, so that its inhibition should ensure an adequate pH for calcium ions transport through the channels, having as result vasodilation. This double mechanism could explain the hypotensive effect of verapamil and amlodipine, with a mechanism that partially dependent on CA I inhibition. Kluwer Academic Publishers, 2000 calcium channel blockers nationallicence verapamil nationallicence amlodipine nationallicence vascular smooth muscle CA I nationallicence pH nationallicence mechanism of action nationallicence Puscas Ioan University of Medicine Oradea, Simleu Silvaniei, 37 Dunarii Street, 4775, Salaj, Romania aut Gilau Ludovic University of Medicine Oradea, Simleu Silvaniei, 37 Dunarii Street, 4775, Salaj, Romania aut Coltau Marcela Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut Pasca Radu Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut Domuta Gabriela Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut Baican Michael Klinik Wilkenberg, Germany aut Hecht Alex Medical Care Unit, Staten Island, New York, USA aut Cardiovascular Drugs and Therapy Kluwer Academic Publishers 14/5(2000-10-01), 523-528 0920-3206 14:5<523 2000 14 10557 https://doi.org/10.1023/A:1007893207279 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007893207279 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Puscas Ioan University of Medicine Oradea, Simleu Silvaniei, 37 Dunarii Street, 4775, Salaj, Romania aut NATIONALLICENCE 700 1- Gilau Ludovic University of Medicine Oradea, Simleu Silvaniei, 37 Dunarii Street, 4775, Salaj, Romania aut NATIONALLICENCE 700 1- Coltau Marcela Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut NATIONALLICENCE 700 1- Pasca Radu Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut NATIONALLICENCE 700 1- Domuta Gabriela Center for Research and Medical Assistance Simleu Silvaniei, Romanian Medical Academy of Sciences, Romania aut NATIONALLICENCE 700 1- Baican Michael Klinik Wilkenberg, Germany aut NATIONALLICENCE 700 1- Hecht Alex Medical Care Unit, Staten Island, New York, USA aut NATIONALLICENCE 773 0- Cardiovascular Drugs and Therapy Kluwer Academic Publishers 14/5(2000-10-01), 523-528 0920-3206 14:5<523 2000 14 10557 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer