caa a22 4500 475834763 CHVBK 20180406123850.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1023/A:1009998304177 doi (NATIONALLICENCE)springer-10.1023/A:1009998304177 The effects of traits of Bacillus megaterium onseed and root colonization and their correlation withthe suppression of Rhizoctonia root rot of soybean [Elektronische Daten] [X. Zheng, J. Sinclair] Bacillus megaterium strainB153-2-2 is a potential bacterial biocontrol agentagainst Rhizoctonia solani isolate 2B12(ISG-2B). To study the role of antagonism (Ant),chemotaxis (Che), motility (Mot), and sporulation(Spo) of the biocontrol agent during seed and rootcolonization and the correlation between rootcolonization and the suppression of soybean (Glycine max) root rot caused by R. solani,strain B153-2-2(Che+Mot+Ant++Spo++) and the sevenderived mutants with altered antagonism, chemotaxis,motility, and/or sporulation were used. The bacterialcells were introduced into soil separately either asa soybean seed coating or soil application. Two soilmixtures defined as coarse and fine soil were used. The bacterial cell chemotactic response to soybeanroot and seed exudates and antagonism to R.solani were significantly (p = 0.05) correlatedwith root and seed colonization in some but not alltreatments. The sporulation-defective mutants had lowcell populations immediately after application and,therefore, reduced root colonization. The differencesin root colonization diminished among the mutants andstrain B153-2-2 when R. solani was present inthe soil or, as seedlings grew older. Soybean seedlingroots grown in coarse soil had significantly greatercolonization by B153-2-2 or its mutants and a lowerdisease index than that in fine soil. There was asignificant positive correlation (r 2 = 0.78)between root colonization by strain B153-2-2 or itsmutants and suppression of Rhizoctonia root rot. Kluwer Academic Publishers, 2000 antagonism nationallicence biocontrol nationallicence chemotaxis nationallicence motility nationallicence soybean nationallicence sporulation nationallicence Bacillus megaterium nationallicence Rhizoctoria solani nationallicence Zheng X. Department of Crop Sciences, University of Illinois at, Urbana-Champaign, Illinois, USA aut Sinclair J. Department of Crop Sciences, University of Illinois at, Urbana-Champaign, Illionis, USA aut BioControl Kluwer Academic Publishers 45/2(2000-06-01), 223-243 1386-6141 45:2<223 2000 45 10526 https://doi.org/10.1023/A:1009998304177 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1009998304177 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng X. Department of Crop Sciences, University of Illinois at, Urbana-Champaign, Illinois, USA aut NATIONALLICENCE 700 1- Sinclair J. Department of Crop Sciences, University of Illinois at, Urbana-Champaign, Illionis, USA aut NATIONALLICENCE 773 0- BioControl Kluwer Academic Publishers 45/2(2000-06-01), 223-243 1386-6141 45:2<223 2000 45 10526 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer