caa a22 4500 475835328 CHVBK 20180406123851.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/BF02515160 doi (NATIONALLICENCE)springer-10.1007/BF02515160 Properties of the charibdotoxin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pig Taenia Coli [Elektronische Daten] [A. Povtyan, A. Zima, M. Harhun, M. Shuba] Using the voltage-clamp technique, we investigated transmembrane ion currents in isolated smooth muscle cells of the guinea pigtaenia coli. In our study, we identified and studied a charibdotoxin-sensitive component of Ca2+-dependent K+ current carried through the channels of high conductance (in most publications called "big conductance,”I BK(Ca)). This component was completely blocked by 100 nM charibdotoxin and by tetraethylammonium in concentrations as low as 1 mM.I BK(Ca) demonstrated fast kinetics of inactivation, which nearly coincided with that of Ca2+ current. In addition to the dependence on Ca2+ concentration, this current also showed voltage-dependent properties: with a rise in the level of depolarization its amplitude increased. In many cells, depolarizing shifts in the membrane potential evoke spontaneous outward currents. Such currents probably represent the secondary effect of cyclic Ca2+ release from the caffeine-sensitive intracellular stores that result in short-term activation of charibdotoxin-sensitive Ca2+-dependent K+ channels. Kluwer Academic/Plenum Publishers, 2000 smooth muscle cells nationallicence Ca2+-dependent K+ channels nationallicence charibdotoxin nationallicence spontaneous outward currents nationallicence Povtyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Harhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/1(2000-01-01), 1-7 0090-2977 32:1<1 2000 32 11062 https://doi.org/10.1007/BF02515160 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02515160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Povtyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Harhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/1(2000-01-01), 1-7 0090-2977 32:1<1 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer