caa a22 4500 475835409 CHVBK 20180406123851.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/BF02515162 doi (NATIONALLICENCE)springer-10.1007/BF02515162 Effects of mibefradil on synaptic transmission in the hippocampus and on voltage-dependent currents in isolated hippocampal and thalamic neurons of the rat [Elektronische Daten] [Yu. Kirichok, Ya. Boychuk, M. Kopanitsa, T. Tsintsadze, N. Lozovaya, O. Krishtal] The effects of a novel anti-hypertensive drug, mibefradil, on voltage-dependent currents in isolated thalamic and hippocampal neurons, as well as on synaptic transmission in the hippocampus have been studied. Mibefradil exerted a potent inhibitory action on low-threshold calcium currents in thalamic neurons (IC50=160 nM). In higher concentrations (1-20 μM), this drug blocked not only low-threshold calcium current but also voltage-dependent sodium and delayed potassium currents in pyramidal hippocampal neurons. The amplitude of population action potentials in hippocampal slices decreased by 55% in the presence of 20μM mibefradil. All of the effects of mibefradil were almost completely reversible. In our experiments, the sensitivity of low-threshold calcium channels in thalamic neurons to mibefradil was higher than that observed on other objects. The ability of mibefradil to block not only calcium currents but also other types of voltage-dependent ion conductances in hippocampal neurons may be considered an essential factor that determines the specificity of the pharmacological profile of this drug. Kluwer Academic/Plenum Publishers, 2000 mibefradil nationallicence low-threshold calcium channels nationallicence sodium channels nationallicence potassium channels nationallicence synaptic transmission nationallicence hippocampus nationallicence Kirichok Yu Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Boychuk Ya Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Kopanitsa M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Tsintsadze T. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Lozovaya N. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Krishtal O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/1(2000-01-01), 12-16 0090-2977 32:1<12 2000 32 11062 https://doi.org/10.1007/BF02515162 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02515162 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirichok Yu Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Boychuk Ya Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Kopanitsa M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Tsintsadze T. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Lozovaya N. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Krishtal O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/1(2000-01-01), 12-16 0090-2977 32:1<12 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer