caa a22 4500 475835492 CHVBK 20180406123851.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1010492000409 doi (NATIONALLICENCE)springer-10.1023/A:1010492000409 Modeling of Myelination and Demyelination Processes in Cell Culture of the Rat Cerebellum [Elektronische Daten] [E. Kolotushkina, T. Pivneva, L. Sokolova, D. Vasilenko, G. Skibo] We studied the structural features of the myelination process under conditions of culturing of dissociated cerebellar tissue obtained from newborn rats, and we made a comparative analysis of the modifications of the structure of myelin sheaths under the influence of a demyelinating factor - blood serum obtained from patients with multiple sclerosis (MS) in different stages of the disease was added to the medium. Under the conditions of our experiments, the optimum term for the formation of full-value myelinated sheaths in vitro was 26 days of culturing; thus, we used this time interval for studying the demyelinating influence. Addition of the blood serum of MS patients in the remission or acute stages very quickly (in a few hours) evoked significant morphological damage to the myelin sheaths of the axons. In general, the demyelinating effects of the blood serum of acute-stage MS patients were more significant. Plenum Publishing Corporation, 2000 myelin nationallicence demyelination nationallicence multiple sclerosis nationallicence antibodies nationallicence oligodendrocytes nationallicence cerebellum nationallicence dissociated cell culture nationallicence Kolotushkina E. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Pivneva T. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Sokolova L. Bogomolets National Medical University, Ministry of Public Health of Ukraine, Kyiv, Ukraine aut Vasilenko D. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Skibo G. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/6(2000-11-01), 383-389 0090-2977 32:6<383 2000 32 11062 https://doi.org/10.1023/A:1010492000409 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1010492000409 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kolotushkina E. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Pivneva T. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Sokolova L. Bogomolets National Medical University, Ministry of Public Health of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Vasilenko D. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Skibo G. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/6(2000-11-01), 383-389 0090-2977 32:6<383 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer