caa a22 4500 475836197 CHVBK 20180406123853.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1005260225624 doi (NATIONALLICENCE)springer-10.1023/A:1005260225624 Effect of Bicuculline on GABA-Activated Transmembrane Ion Currents in Neurons of the Rat Spinal Ganglia [Elektronische Daten] [V. Sidorenko, N. Veselovsky] We obtained quantitative estimates of the effect exerted by a blocker of GABAA receptors, bicuculline, on GABA-activated transmembrane ion currents in isolated neurons from the rat spinal ganglia. The currents were evoked by short (20 msec) local GABA applications. Increases in the bicuculline concentration from 10 nM to 2 μM progressively decreased the amplitude of GABA-activated ion currents; 5 μM of the blocker completely suppressed these currents, while washing out recovered them. The dependence between the level of bicuculline-induced blockade and concentration of the agent was nonlinear and could be satisfactorily fitted by a reverse logistic function. Within the studied concentration range, bicuculline exerted no influence on the kinetic parameters of the current rising and falling phases. Plenum Publishing Corporation, 2000 GABA nationallicence bicuculline nationallicence ion currents nationallicence isolated neurons nationallicence spinal ganglia nationallicence Sidorenko V. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Veselovsky N. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/4(2000-07-01), 266-270 0090-2977 32:4<266 2000 32 11062 https://doi.org/10.1023/A:1005260225624 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005260225624 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sidorenko V. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Veselovsky N. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/4(2000-07-01), 266-270 0090-2977 32:4<266 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer