caa a22 4500 475836367 CHVBK 20180406123854.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1023/A:1010319305794 doi (NATIONALLICENCE)springer-10.1023/A:1010319305794 Kharchenko N. Inter-Departmental Center of Clinical and Experimental Narcology, Ministry of Public Health, Ukraine aut Effects of Glycine on the Catecholamine Levels and Activities of Alcohol-Metabolizing Enzymes in Rats with Alcohol Intoxication and Addiction [Elektronische Daten] [N. Kharchenko] We studied in rats the effects of peroral glycine introduction on the contents of catecholamines (CA) - noradrenaline (NA) and dopamine (DA) - in different brain structures (hypothalamus, midbrain, and neocortex), as well as the levels of adrenaline (A), NA, and DA in the blood and the activity of alcohol-metabolizing (AlM) enzymes - alcohol dehydrogenase (AlDH) and aldehyde dehydrogenase (AdhDH) - in the blood serum. The experimental group included animals with a disposition to alcohol consumption under conditions of free choice for drinking between an alcohol solution and water. The measurements were performed in animals in the state of acute alcohol intoxication (i.p. injection of 4 g/kg ethanol) or chronic alcohol addiction (formed due to a 3-month-long free access to ethanol solution). Introduction of 150 mg/kg glycine increased the NA and DA contents (the latter, to a lesser extent) in all examined brain structures; the NA level in the blood increased, while that of DA decreased. Under conditions of acute alcohol intoxication and chronic alcohol addiction, the ratio of the activities of AlM enzymes, AdhDH/AlDH, was significantly shifted toward values indicative of accumulation of acetaldehyde (AcAdh) in the tissues. This was accompanied by noticeable modifications of the CA contents in the brain structures and blood of the rats; in particular, the [DA]/[NA] ratio in the brain significantly increased. Introduction of glycine under conditions of acute alcohol intoxication provided normalization of the AdhDH/AlDH activity ratio. Obvious trends toward normalization of the CA levels in the brain structures were also observed in both acute and chronic experiments. In the latter case, the glycine treatment course resulted in a drop in the daily alcohol consumption by the animals. We conclude that glycine, which binds AcAdh and modifies the metabolism of CA transmitters, exerts a significant corrective influence on the pathogenetic mechanisms of alcohol addiction. Our experimental findings demonstrate that there are contact points between the "acetaldehyde” and "catecholamine” hypotheses of pathogenesis of alcoholism. Plenum Publishing Corporation, 2000 neurotransmitters nationallicence alcoholism nationallicence alcohol intoxication and addiction nationallicence alcohol-metabolizing enzymes nationallicence catecholaminergic systems nationallicence Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/5(2000-09-01), 312-320 0090-2977 32:5<312 2000 32 11062 https://doi.org/10.1023/A:1010319305794 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1010319305794 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kharchenko N. Inter-Departmental Center of Clinical and Experimental Narcology, Ministry of Public Health, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/5(2000-09-01), 312-320 0090-2977 32:5<312 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer