caa a22 4500 475836405 CHVBK 20180406123854.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/BF02515170 doi (NATIONALLICENCE)springer-10.1007/BF02515170 Properties of the apamin-sensitive component of Ca2+-dependent K+ current in smooth muscle cells of the guinea pig taenia coli [Elektronische Daten] [A. Povstyan, A. Zima, M. Harhun, M. Shuba] With the help of a standard voltage-clamp technique, we investigated transmembrane ion currents in isolated smooth muscle cells of the guinea pigtaenia coli. In Ca2+-dependent K+ current, we identified and studied the properties of an apamin-sensitive voltage-independent component carried through the channels of low conductance (in many publications called small conductance,I SK(Ca)). This component did not show the temporal inactivation;I SK(Ca) was insensitive to the action of 4 mM tetraethylammonium, but was completely blocked by 500 nM of apamin. It was shown thatI SK(Ca) is very sensitive to changes in the intracellular Ca2+ concentration ([Ca2+] i ): a decrease in [Ca2+] i up to 50 nM resulted in the almost complete blockade of the current. The entry of Ca ions into a cell from the external solution through the voltage-operated Ca2+ channels of L-type was not an obligatory condition for activation ofI SK(Ca). The current-voltage relationship forI SK(Ca) had a maximum within the voltage range of +20 to +50 mV. Kluwer Academic/Plenum Publishers, 2000 smooth muscle cells nationallicence Ca2+-dependent K+ channels nationallicence intracellular Ca2+ concentration nationallicence apamin nationallicence tetraethylammonium nationallicence Povstyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Harhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/2(2000-03-01), 63-69 0090-2977 32:2<63 2000 32 11062 https://doi.org/10.1007/BF02515170 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02515170 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Povstyan A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Zima A. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Harhun M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Shuba M. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Kluwer Academic Publishers-Plenum Publishers 32/2(2000-03-01), 63-69 0090-2977 32:2<63 2000 32 11062 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer