caa a22 4500 475844890 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s000180050501 doi (NATIONALLICENCE)springer-10.1007/s000180050501 Current applications of single-cellPCR [Elektronische Daten] [S. Hahn*, X. Y. Zhong, C. Troeger, R. Burgemeister, K. Gloning, W. Holzgreve] Abstract.: The advent of the polymerase chain reaction (PCR) has revolutionised the way in which molecular biologists view their task at hand, for it is now possible to amplify and examine minute quantities of rare genetic material: the limit of this exploration being the single cell. It is especially in the field of prenatal diagnostics that this ability has been readily seized upon, as it has opened up the prospect of preimplantation genetic analysis and the use of fetal cells enriched from the blood of pregnant women for the assessment of single-gene Mendelian disorders. However, apart from diagnostic applications, single-cell PCR has proven to be of enormous use to basic scientists, addressing diverse immunological, neurological and developmental questions, where both the genome but also messenger RNA expression patterns were examined. Furthermore, recent advances, such as optimised whole genome amplification (WGA) procedures, single-cell complementary DNA arrays and perhaps even single-cell comparative genomic hybridisation will ensure that the genetic analysis of single cells will become common practice, thereby opening up new possibilities for diagnosis and research. Birkhäuser Verlag Basel,, 2000 Key words. PCR nationallicence single cells nationallicence diagnosis nationallicence Hahn* S. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut Zhong X. Y. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut Troeger C. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut Burgemeister R. Pränatal-Medizin München, Frauenärzte und Genetik, Munich (Germany), DE aut Gloning K. Pränatal-Medizin München, Frauenärzte und Genetik, Munich (Germany), DE aut Holzgreve W. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut https://doi.org/10.1007/s000180050501 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050501 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hahn* S. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut NATIONALLICENCE 700 1- Zhong X. Y. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut NATIONALLICENCE 700 1- Troeger C. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut NATIONALLICENCE 700 1- Burgemeister R. Pränatal-Medizin München, Frauenärzte und Genetik, Munich (Germany), DE aut NATIONALLICENCE 700 1- Gloning K. Pränatal-Medizin München, Frauenärzte und Genetik, Munich (Germany), DE aut NATIONALLICENCE 700 1- Holzgreve W. Laboratory for Prenatal Medicine, Department of Obstetrics and Gynaecology, University of Basel, Schanzenstrasse 46, CH-4031 Basel (Switzerland), Fax +41 61 325 9399, e-mail: shahn@uhbs.ch, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer