caa a22 4500 475844947 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s000180050503 doi (NATIONALLICENCE)springer-10.1007/s000180050503 Catalano C. E. Department of Pharmaceutical Sciences and the Molecular Biology Program, University of Colorado Health Sciences Center, 4200 East Ninth Avenue Denver (Colorado 80262, USA), Fax +1 303 315 6281, e-mail: carlos.catalano@uchsc.edu, US aut The terminase enzyme from bacteriophage lambda: a DNA-packaging machine [Elektronische Daten] [C. E. Catalano] Abstract.: This review focuses on the biochemical, biophysical, and catalytic properties of terminase, an enzyme involved in bacteriophage λ genome packaging. The holoenzyme possesses ATPase, DNA strand-separation, and site-specific nuclease activities that work in concert to insert a viral genome into the confines of a preformed capsid. Moreover, the terminase subunits are part of a series of nucleoprotein complexes involved in genome packaging, including remarkably stable intermediates that transition to a highly mobile DNA packaging ‘machine.' Models for the assembly and interconversion of these complexes are presented. Interactions between the catalytic sites in the enzyme complex, and modulation of these catalytic activities as it relates to the assembly and relative stability of the packaging intermediates are discussed. This ordered progression of nucleoprotein intermediates is a common theme in biology as demonstrated by mechanistic similarities between viral DNA packaging, the initiation of chromosomal replication, and the initiation of transcription. Terminase is thus part of a growing number of examples of biological ‘machines' or molecular ‘motors.' Birkhäuser Verlag Basel,, 2000 Key words. Phage lambda terminase nationallicence DNA packaging nationallicence virus assembly nationallicence molecular motors nationallicence https://doi.org/10.1007/s000180050503 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050503 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Catalano C. E. Department of Pharmaceutical Sciences and the Molecular Biology Program, University of Colorado Health Sciences Center, 4200 East Ninth Avenue Denver (Colorado 80262, USA), Fax +1 303 315 6281, e-mail: carlos.catalano@uchsc.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer