caa a22 4500 475844955 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s000180050507 doi (NATIONALLICENCE)springer-10.1007/s000180050507 Muscarinic M3 receptor subtype gene expression in the human heart [Elektronische Daten] [I. Hellgren*, A. Mustafa, M. Riazi, I. Suliman, C. Sylvén, A. Adem] Abstract.: The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M2 and M3 subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M1 receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M2 and M3 subtypes. This is the first report of M3 receptors in the human myocardium. Birkhäuser Verlag Basel,, 2000 Key words. Muscarinic receptor nationallicence subtypes nationallicence human nationallicence heart failure nationallicence ventricle nationallicence atrium nationallicence mRNA nationallicence protein nationallicence Hellgren* I. Department of Cardiology, The Clinical Research Center, 5th floor, NOVUM, Karolinska Institute, Huddinge University Hospital, 141 86 Huddinge (Sweden), Fax +46 8 58583810, e-mail: irina.hellgren@medhs.ki.se, SE aut Mustafa A. Department of Clinical Neuroscience, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut Riazi M. Department of Orthopedic Surgery, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut Suliman I. Department of Orthopedic Surgery, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut Sylvén C. Department of Cardiology, The Clinical Research Center, 5th floor, NOVUM, Karolinska Institute, Huddinge University Hospital, 141 86 Huddinge (Sweden), Fax +46 8 58583810, e-mail: irina.hellgren@medhs.ki.se, SE aut Adem A. Department of Clinical Neuroscience, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut https://doi.org/10.1007/s000180050507 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050507 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hellgren* I. Department of Cardiology, The Clinical Research Center, 5th floor, NOVUM, Karolinska Institute, Huddinge University Hospital, 141 86 Huddinge (Sweden), Fax +46 8 58583810, e-mail: irina.hellgren@medhs.ki.se, SE aut NATIONALLICENCE 700 1- Mustafa A. Department of Clinical Neuroscience, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut NATIONALLICENCE 700 1- Riazi M. Department of Orthopedic Surgery, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut NATIONALLICENCE 700 1- Suliman I. Department of Orthopedic Surgery, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut NATIONALLICENCE 700 1- Sylvén C. Department of Cardiology, The Clinical Research Center, 5th floor, NOVUM, Karolinska Institute, Huddinge University Hospital, 141 86 Huddinge (Sweden), Fax +46 8 58583810, e-mail: irina.hellgren@medhs.ki.se, SE aut NATIONALLICENCE 700 1- Adem A. Department of Clinical Neuroscience, Karolinska Institute, Huddinge University Hospital, Stockholm (Sweden), SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer