caa a22 4500 475844963 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/s000180050506 doi (NATIONALLICENCE)springer-10.1007/s000180050506 Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats [Elektronische Daten] [A. Mate, M. A. de la Hermosa, A. Barfull, I. Sánchez-Aguayo, J. M. Planas, C. M. Vázquez*] Abstract.: Na+-dependent D-glucose and D-galactose transport were studied in brush-border membrane vesicles (BBMVs) from kidney cortex isolated from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of Na+-dependent D-glucose and D-galactose transport were significantly lower in SHR compared with WKY, the observed decreases being similar for both substrates. To explain the reduction in sugar transport by renal BBMVs, the density of Na+-dependent sugar cotransporters was studied in BBMVs from kidney cortex isolated from SHR and WKY rats. Phlorizin-specific binding and Western blot analysis indicated a reduction in the density of the cotransporters in SHR relative to WKY rats. This reduction was similar to those found for the initial rates and accumulation ratios for D-glucose and D-galactose in SHR. Na+ uptake, studied using 22Na+, was significantly increased in SHR, so the observed reduction in sugar transport could be due to disruption of the Na+ gradient between renal BBMVs in SHR. Furthermore, a significant decrease in the activity of Na+-K+-ATPase was observed in SHR. In conclusion, changes in the density of the Na+-dependent sugar cotransporter and in the Na+ gradient across the brush-border membranes might be involved in the observed reduction in sugar transport by renal BBMVs from SHR. Birkhäuser Verlag Basel,, 2000 Key words. Sugar transport nationallicence SHR nationallicence kidney nationallicence rat nationallicence BBMV nationallicence Mate A. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut de la Hermosa M. A. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut Barfull A. Departamento de Fisiologia-Divisió IV, Facultad de Farmacia, Universidad de Barcelona, Avda Joan XXIII s/n, E-08028 Barcelona (Spain), ES aut Sánchez-Aguayo I. Departamento de Biologı´a Celular, Facultad de Biologı´a, Universidad de Sevilla, Avda Reina Mercedes s/n, E-41012 Sevilla (Spain), ES aut Planas J. M. Departamento de Fisiologia-Divisió IV, Facultad de Farmacia, Universidad de Barcelona, Avda Joan XXIII s/n, E-08028 Barcelona (Spain), ES aut Vázquez* C. M. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut https://doi.org/10.1007/s000180050506 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050506 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mate A. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut NATIONALLICENCE 700 1- de la Hermosa M. A. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut NATIONALLICENCE 700 1- Barfull A. Departamento de Fisiologia-Divisió IV, Facultad de Farmacia, Universidad de Barcelona, Avda Joan XXIII s/n, E-08028 Barcelona (Spain), ES aut NATIONALLICENCE 700 1- Sánchez-Aguayo I. Departamento de Biologı´a Celular, Facultad de Biologı´a, Universidad de Sevilla, Avda Reina Mercedes s/n, E-41012 Sevilla (Spain), ES aut NATIONALLICENCE 700 1- Planas J. M. Departamento de Fisiologia-Divisió IV, Facultad de Farmacia, Universidad de Barcelona, Avda Joan XXIII s/n, E-08028 Barcelona (Spain), ES aut NATIONALLICENCE 700 1- Vázquez* C. M. Departamento de Fisiologı´a y Biologı´a Animal, Facultad de Farmacia, Universidad de Sevilla, C/Tramontana s/n, E-41012 Sevilla (Spain), Fax +34954233765, e-mail: vazquez@fafar.us.es, ES aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer