caa a22 4500 475845005 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/PL00000676 doi (NATIONALLICENCE)springer-10.1007/PL00000676 Polysialic acids: potential in improving the stability and pharmacokinetics of proteins and other therapeutics [Elektronische Daten] [G. Gregoriadis*, A. Fernandes**, M. Mital, B. McCormack] Abstract.: Naturally occurring polymers of N-acetylneuraminic acid (polysialic acids) are biodegradable, highly hydrophilic and have no known receptors in the body. Following intravenous injection, polysialic acids exhibit long half-lives in the blood circulation and have therefore been proposed as carriers of short-lived drugs and small peptides. In addition, shorter-chain polysialic acids can be used as a means to increase the circulatory half-life of proteins and thus serve as an alternative to the nonbiodegradable monomethoxypoly(ethylene glycol). Recent work has shown that covalent coupling of a low molecular weight polysialic acid (colominic acid) to catalase and asparaginase leads to a considerable increase of enzyme stability in the presence of proteolytic enzymes or blood plasma. Comparative studies in vivo with polysialylated and intact asparaginase revealed that polysialylation significantly increases the half-life of the enzyme. The highly hydrophilic and innocuous nature of polysialic acids renders them suitable as a means to prolong the circulation of peptides and proteins. Birkhäuser Verlag Basel,, 2000 Key words. Polysialic acids nationallicence colominic acid nationallicence catalase nationallicence asparaginase nationallicence Gregoriadis* G. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut Fernandes** A. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut Mital M. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut McCormack B. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut https://doi.org/10.1007/PL00000676 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000676 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gregoriadis* G. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut NATIONALLICENCE 700 1- Fernandes** A. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut NATIONALLICENCE 700 1- Mital M. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut NATIONALLICENCE 700 1- McCormack B. Centre for Drug Delivery Research School of Pharmacy, University of London, 29-39 Brunswick Square, London WC1N 1AX (United Kingdom), Fax +44 171 7535822, e-mail: gregoriadis@cua.ulsop.ac.uk, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer