caa a22 4500 475845056 CHVBK 20180406123911.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/PL00000672 doi (NATIONALLICENCE)springer-10.1007/PL00000672 Ferrochelatase at the millennium: structures, mechanisms and [2Fe-2S] clusters [Elektronische Daten] [H. A. Dailey*, T. A. Dailey, C.-K. Wu**, A. E. Medlock, J. P. Rose, K.-F. Wang] Abstract.: Ferrochelatase (E.C. 4.99.1.1, protoheme ferrolyase) catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme (heme). In the past 2 years, the crystal structures of ferrochelatases from the bacterium Bacillus subtilis and human have been determined. These structures along with years of biophysical and kinetic studies have led to a better understanding of the catalytic mechanism of ferrochelatase. At present, the complete DNA sequences of 45 ferrochelatases from procaryotes and eucaryotes are available. These sequences along with direct protein studies reveal that ferrochelatases, while related, vary significantly in amino acid sequence, molecular size, subunit composition, solubility, and the presence or absence of nitric-oxide-sensitive [2Fe-2S] cluster. Birkhäuser Verlag Basel,, 2000 Key words. Ferrochelatase nationallicence heme synthesis nationallicence iron sulfur cluster nationallicence metallation nationallicence porphyria nationallicence Dailey* H. A. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Dailey T. A. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Wu** C.-K Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Medlock A. E. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Rose J. P. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Wang K.-F Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut https://doi.org/10.1007/PL00000672 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000672 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dailey* H. A. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut NATIONALLICENCE 700 1- Dailey T. A. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut NATIONALLICENCE 700 1- Wu** C.-K Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut NATIONALLICENCE 700 1- Medlock A. E. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut NATIONALLICENCE 700 1- Rose J. P. Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut NATIONALLICENCE 700 1- Wang K.-F Department of Biochemistry and Molecular Biology, and Department of Microbiology, University of Georgia, Athens (Georgia 30605-7229, USA), Fax +1 706 542 7567, e-mail: hdailey@arches.uga.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer