caa a22 4500 47584517X CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/PL00000731 doi (NATIONALLICENCE)springer-10.1007/PL00000731 Adducin: structure, function and regulation [Elektronische Daten] [Y. Matsuoka*, X. Li, V. Bennett] Abstract.: Adducin is a ubiquitously expressed membrane-skeletal protein localized at spectrin-actin junctions that binds calmodulin and is an in vivo substrate for protein kinase C (PKC) and Rho-associated kinase. Adducin is a tetramer comprised of either α/β or α/γ heterodimers. Adducin subunits are related in sequence and all contain an N-terminal globular head domain, a neck domain and a C-terminal protease-sensitive tail domain. The tail domains of all adducin subunits end with a highly conserved 22-residue myristoylated alanine-rich C kinase substrate (MARCKS)-related domain that has homology to MARCKS protein. Adducin caps the fast-growing ends of actin filaments and also preferentially recruits spectrin to the ends of filaments. Both the neck and the MARCKS-related domains are required for these activities. The neck domain self-associates to form oligomers. The MARCKS-related domain binds calmodulin and contains the major phosphorylation site for PKC. Calmodulin, gelsolin and phosphorylation by the kinase inhibit in vitro activities of adducin involving actin and spectrin. Recent observations suggest a role for adducin in cell motility, and as a target for regulation by Rho-dependent and Ca2+-dependent pathways. Prominent physiological sites of regulation of adducin include dendritic spines of hippocampal neurons, platelets and growth cones of axons. Birkhäuser Verlag Basel,, 2000 Key words. Membrane skeleton nationallicence adducin nationallicence spectrin nationallicence actin nationallicence Matsuoka* Y. Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan), Fax +81 3 3542 3586, e-mail: yomatsuo@gan2.res.ncc.go.jp, JP aut Li X. Department of Pharmacology and Cancer Biology, and Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham (North Carolina 27710, USA), US aut Bennett V. Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan), Fax +81 3 3542 3586, e-mail: yomatsuo@gan2.res.ncc.go.jp, JP aut https://doi.org/10.1007/PL00000731 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000731 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsuoka* Y. Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan), Fax +81 3 3542 3586, e-mail: yomatsuo@gan2.res.ncc.go.jp, JP aut NATIONALLICENCE 700 1- Li X. Department of Pharmacology and Cancer Biology, and Howard Hughes Medical Institute and Department of Cell Biology, Duke University Medical Center, Durham (North Carolina 27710, USA), US aut NATIONALLICENCE 700 1- Bennett V. Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045 (Japan), Fax +81 3 3542 3586, e-mail: yomatsuo@gan2.res.ncc.go.jp, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer