caa a22 4500 475845277 CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/PL00000738 doi (NATIONALLICENCE)springer-10.1007/PL00000738 Decreased pulmonary levels of the anti-inflammatory Clara cell 16 kDa protein after induction of airway inflammation in asthmatics [Elektronische Daten] [C. Lensmar*, M. Nord, G. H. Gudmundsson, A. Roquet, O. Andersson, H. Jörnvall, A. Eklund, J. Grunewald, B. Agerberth] Abstract.: The Clara cell 16 kDa protein (CC16) maps to an atopy-associated region of chromosome 11 and has been ascribed an anti-inflammatory function. Using reverse-phase HPLC and Western blot analysis, we have evaluated the polypeptide pattern in bronchoalveolar lavage (BAL) fluid retrieved from asthmatics, before and after induction of airway inflammation by low-dose allergen inhalation challenge. A prominent decrease of CC16 was seen after induction of inflammation, and a further CC16 decrease was observed in lavage fluid where surfactant had been removed. Reduced levels of pulmonary CC16 may cause loss of anti-inflammatory activity in the airways and contribute to the development of airway inflammation in asthma. Birkhäuser Verlag Basel,, 2000 Key words. CC16 nationallicence reverse-phase HPLC nationallicence asthma nationallicence BAL fluid proteins nationallicence Lensmar* C. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut Nord M. Department of Medical Nutrition, Novum, Huddinge University Hospital, S-141 86 Huddinge (Sweden), SE aut Gudmundsson G. H. Microbiology and Tumorbiology Center, and, SE aut Roquet A. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut Andersson O. Department of Medical Nutrition, Novum, Huddinge University Hospital, S-141 86 Huddinge (Sweden), SE aut Jörnvall H. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm (Sweden), SE aut Eklund A. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut Grunewald J. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut Agerberth B. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm (Sweden), SE aut https://doi.org/10.1007/PL00000738 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000738 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lensmar* C. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut NATIONALLICENCE 700 1- Nord M. Department of Medical Nutrition, Novum, Huddinge University Hospital, S-141 86 Huddinge (Sweden), SE aut NATIONALLICENCE 700 1- Gudmundsson G. H. Microbiology and Tumorbiology Center, and, SE aut NATIONALLICENCE 700 1- Roquet A. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut NATIONALLICENCE 700 1- Andersson O. Department of Medical Nutrition, Novum, Huddinge University Hospital, S-141 86 Huddinge (Sweden), SE aut NATIONALLICENCE 700 1- Jörnvall H. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm (Sweden), SE aut NATIONALLICENCE 700 1- Eklund A. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut NATIONALLICENCE 700 1- Grunewald J. Department of Medicine, Division of Respiratory Medicine, Lung Research Laboratory, L2:01 Karolinska Hospital, S-171 76 Stockholm (Sweden), Fax +46 8 51 77 54 51, e-mail: catarinal@lung.ks.se, SE aut NATIONALLICENCE 700 1- Agerberth B. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm (Sweden), SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer