caa a22 4500 475845331 CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00000748 doi (NATIONALLICENCE)springer-10.1007/PL00000748 Ceramides induce apoptosis in HeLa cells and enhance cytochrome c-induced apoptosis in Xenopus egg extracts [Elektronische Daten] [Z. Jiang, X. Hong, H. Long, J. Hu, Z. Zhai*] Abstract.: Ceramide has been reported to induce typical apoptotic changes in nuclei incubated in a cell-free system, and that the addition of ceramide bypasses the requirement for mitochondria. Here, we explore the possible pathways by which ceramide induces apoptosis either in intact cells or in a cell-free system which we have developed. We found that in the cell-free system, C2-ceramide is not able to induce apoptosis in nuclei whereas cytochrome c does, but it is able to induce HeLa cells to undergo apoptosis. Ceramide is also not able to induce apoptosis when added into the cell-free system together with purified mitochondria. Further investigation showed that C2-ceramide at certain concentrations greatly increases nuclear apoptosis caused by cytochrome c in the cell-free system. From these results we conclude that the induction of apoptosis by ceramide may require intact cells in which some unknown signal transduction pathways are involved. Birkhäuser Verlag Basel,, 2000 Key words. Ceramide nationallicence HeLa cell nationallicence apoptosis nationallicence egg extract nationallicence cytochrome c nationallicence nuclei nationallicence Jiang Z. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut Hong X. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut Long H. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut Hu J. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut Zhai* Z. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut https://doi.org/10.1007/PL00000748 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000748 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Z. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut NATIONALLICENCE 700 1- Hong X. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut NATIONALLICENCE 700 1- Long H. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut NATIONALLICENCE 700 1- Hu J. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut NATIONALLICENCE 700 1- Zhai* Z. Department of Cell Biology and Genetics, The College of Life Sciences, Peking University, Beijing 100871 (China), Fax +86 10 62751526, e-mail: zhaizh@plum.lsc.pku.edu.cn, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer