caa a22 4500 475845374 CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00000746 doi (NATIONALLICENCE)springer-10.1007/PL00000746 Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells [Elektronische Daten] [H. Kanouchi, T. Oka, K. Asagi, H. Tachibana, K. Yamada*] Abstract.: To clarify the biological role of kidney perchloric acid-soluble protein 1 (K-PSP1), its expression and intracellular distribution were examined in normal rat kidney epithelial NRK-52E cells. K-PSP1 expression was low during the proliferating phase and high in the stationary phase, and shown to have a negative relationship with the protein-synthesizing activity of the cells. Immunocytochemical studies revealed that K-PSP1 is predominantly located in the cytosol, especially in endoplasmic reticulum and Golgi apparatus of proliferating cells. In the stationary phase, K-PSP1 was not detected immunologically even though protein and mRNA expression were high. This disappearance of reactivity with anti-serum seems to be due to a conformational change in K-PSP1 induced by unknown factors. These results suggest that the role of K-PSP1 is to regulate cell proliferation, and this may be related to a previously reported ability to inhibit protein synthesis. Birkhäuser Verlag Basel,, 2000 Key words. Perchlolic acid-soluble protein nationallicence protein synthesis inhibitor nationallicence cell growth nationallicence immunoblotting nationallicence quantitative RT-PCR reaction nationallicence immunofluorescent cytochemistry nationallicence Kanouchi H. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut Oka T. Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065 (Japan), JP aut Asagi K. Otsuka Pharmaceutical Factory, Inc., Nutrition Research Institute, 115 Aza Kuguhara Tateiwa, Muya-cho, Naruto-shi, Tokushima 772-8601 (Japan), JP aut Tachibana H. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut Yamada* K. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut https://doi.org/10.1007/PL00000746 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000746 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanouchi H. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut NATIONALLICENCE 700 1- Oka T. Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065 (Japan), JP aut NATIONALLICENCE 700 1- Asagi K. Otsuka Pharmaceutical Factory, Inc., Nutrition Research Institute, 115 Aza Kuguhara Tateiwa, Muya-cho, Naruto-shi, Tokushima 772-8601 (Japan), JP aut NATIONALLICENCE 700 1- Tachibana H. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut NATIONALLICENCE 700 1- Yamada* K. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Graduate School of Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 (Japan), Fax +81 92 642 3007, e-mail: yamadako@agr.kyushu-u.ac.jp, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer