caa a22 4500 475845382 CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00000747 doi (NATIONALLICENCE)springer-10.1007/PL00000747 Differential stimulation of signaling pathways initiated by Edg-2 in response to lysophosphatidic acid or sphingosine-1-phosphate [Elektronische Daten] [**, O. Peyruchaud*, D. F. Mosher] Abstract.: Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are produced during cell activation and have multiple effects on cells. A family of seven transmembrane-spanning domain G-protein-coupled receptors, named Edg, mediate these effects of LPA and S1P. In this study, transient overexpression of Edg-2 sensitized MG63 human osteosarcoma cells to both LPA- and S1P-mediated stimulation of fibronectin matrix deposition and actin stress fiber formation. Both lipids were active in the 1-20 nM concentration range on cells transfected with Edg-2 as compared to the 10-200 nM range on mock-transfected cells. The signaling pathway for matrix deposition by Edg-2-transfected cells was Rho dependent. Overexpression of Edg-2 also caused a tenfold decrease in the concentration of either LPA or S1P that activated MAPKinase (Erk1/2) in MG63 cells. LPA- or S1P-stimulated activation of Erkl/2 was Gi dependent. These results indicate that, in MG63 cells, Edg-2 mediates actin stress fiber formation, fibronectin matrix assembly, and MAPKinase activation in response to either LPA or S1P. Birkhäuser Verlag Basel,, 2000 Key words. MG63 cells nationallicence Edg-2 nationallicence LPA nationallicence S1P nationallicence fibronectin nationallicence MAPKinase nationallicence GFP nationallicence Rho nationallicence Peyruchaud* **, O. Department of Medicine, University of Wisconsin-Madison, 1300 University Avenue, Madison (Wisconsin 53706, USA), US aut Mosher D. F. Department of Medicine, University of Wisconsin-Madison, 1300 University Avenue, Madison (Wisconsin 53706, USA), US aut https://doi.org/10.1007/PL00000747 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000747 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peyruchaud* **, O. Department of Medicine, University of Wisconsin-Madison, 1300 University Avenue, Madison (Wisconsin 53706, USA), US aut NATIONALLICENCE 700 1- Mosher D. F. Department of Medicine, University of Wisconsin-Madison, 1300 University Avenue, Madison (Wisconsin 53706, USA), US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer