caa a22 4500 475845404 CHVBK 20180406123912.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/PL00000749 doi (NATIONALLICENCE)springer-10.1007/PL00000749 Kinetics of BRCA1 regulation in response to UVC radiation [Elektronische Daten] [C. E. K. Clarkin, H. Zhang, B. L. Weber*] Abstract.: To investigate changes in BRCA1 following DNA damage, we exposed MCF-7 cells to increasing doses of ultraviolet C. We observed an increase in BRCA1 protein levels above 78 J/m2. This increase was observed as early as 5 min after irradiation. BRCA1 levels were then observed to decrease after 2 h, consistent with the previously published data. By pretreating with cycloheximide prior to irradiation, we observed a decrease in the protein half-life, from 3.5 h to 53 min, suggesting that a decrease in protein half-life may cause the lower levels of BRCA1 after irradiation. We also observed an increase in BRCA1 mRNA within 15 min of irradiation, followed by a decrease after 4 h. These data suggest that newly translated protein may contribute to increases in BRCA1 protein levels. The very rapid changes in BRCA1 support its role as a sensor of DNA damage, as opposed to being a repair gene. Birkhäuser Verlag Basel,, 2000 Key words. Breast cancer nationallicence BRCA1 nationallicence DNA damage nationallicence ultraviolet C nationallicence MCF-7 nationallicence Clarkin C. E. K. Department of Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), US aut Zhang H. Department of Medicine, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), Fax +1 215 573 2486, e-mail: weberb@mail.med.upenn.edu, US aut Weber* B. L. Department of Medicine, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), Fax +1 215 573 2486, e-mail: weberb@mail.med.upenn.edu, US aut https://doi.org/10.1007/PL00000749 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000749 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clarkin C. E. K. Department of Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), US aut NATIONALLICENCE 700 1- Zhang H. Department of Medicine, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), Fax +1 215 573 2486, e-mail: weberb@mail.med.upenn.edu, US aut NATIONALLICENCE 700 1- Weber* B. L. Department of Medicine, University of Pennsylvania, Philadelphia (Pennsylvania 19104, USA), Fax +1 215 573 2486, e-mail: weberb@mail.med.upenn.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer