caa a22 4500 475845528 CHVBK 20180406123913.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/PL00000659 doi (NATIONALLICENCE)springer-10.1007/PL00000659 Diet influences the colonisation of Campylobacter jejuni and distribution of mucin carbohydrates in the chick intestinal tract [Elektronische Daten] [F. Fernandez*, R. Sharma, M. Hinton, M. R. Bedford] Abstract.: The objectives of this study were to evaluate the effect of diet on the colonisation by Campylobacter jejuni of the chick caeca, and to determine whether the viscosity of the intestinal contents and mucin carbohydrates were altered by the diet. The diets investigated were maize based, wheat based or wheat-based supplemented with xylanase. The xylanase-supplemented diet reduced the viscosity and lowered the numbers of Camp. jejuni. Feeding the enzyme-supplemented diet increased the amount of neutral and sulphated mucins in the goblet cells of the small and large intestines and caecum. An abundance of sulphated and carboxylated mucins was seen in the surface goblet cells of the large intestine with the maize- and wheat-based diets. Both the diet supplemented with xylanase and the maize diets increased crypt-surface glycosylation of the sialic acid residues. The analysed data from the combined sites showed significant differences in the amount of neutral and acidic mucins when comparing the wheat and the wheat plus xylanase diets. However, no changes were shown in the staining intensity of sulphated mucins between the three diets. Significant differences in the glycosylation of sialic acid and in the N-acetylglucosamine residues were shown between dietary groups. These results provide evidence that the wheat diet supplemented with xylanase leads to greater changes in the mucin composition and carbohydrate expression of goblet cell glycoconjugates, which are associated with a reduction in intestinal viscosity and decreased numbers of Camp. jejuni. Birkhäuser Verlag Basel,, 2000 Key words.Campylobacter nationallicence chick nationallicence intestine nationallicence mucin nationallicence xylanase nationallicence Fernandez* F. Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset, Bristol, BS40 5DU (UK), Fax +44 1934 853145, e-mail: fresie.fernandez@bristol.ac.uk, UA aut Sharma R. Human Morphology, University of Southampton, Basset Crescent East, Southampton, SO16 7PX (UK), UA aut Hinton M. Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset, Bristol, BS40 5DU (UK), Fax +44 1934 853145, e-mail: fresie.fernandez@bristol.ac.uk, UA aut Bedford M. R. Finnfeeds International Ltd, Ailesbury Court, High Street, Marlborough, Wiltshire, SN8 1AA (UK), UA aut https://doi.org/10.1007/PL00000659 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000659 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernandez* F. Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset, Bristol, BS40 5DU (UK), Fax +44 1934 853145, e-mail: fresie.fernandez@bristol.ac.uk, UA aut NATIONALLICENCE 700 1- Sharma R. Human Morphology, University of Southampton, Basset Crescent East, Southampton, SO16 7PX (UK), UA aut NATIONALLICENCE 700 1- Hinton M. Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, North Somerset, Bristol, BS40 5DU (UK), Fax +44 1934 853145, e-mail: fresie.fernandez@bristol.ac.uk, UA aut NATIONALLICENCE 700 1- Bedford M. R. Finnfeeds International Ltd, Ailesbury Court, High Street, Marlborough, Wiltshire, SN8 1AA (UK), UA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer