caa a22 4500 475845692 CHVBK 20180406123913.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/PL00000768 doi (NATIONALLICENCE)springer-10.1007/PL00000768 Differential basal synthesis of Hsp70/Hsc70 contributes to interindividual variation in Hsp70/Hsc70 inducibility [Elektronische Daten] [T. Boshoff, F. Lombard, R. Eiselen, J. J. Bornman, M. Bachelet, B. S. Polla, L. Bornman*] Abstract.: The source of intraspecies variation in the expression of heat shock proteins (HSPs) remains unresolved but could shed light on differential stress tolerance and disease susceptibility. This study investigated the influence of variable basal HSP synthesis on differential inducibility of HSP synthesis. Basal and heat-induced synthesis of the major HSP families in peripheral blood monocytes from healthy donors (n=42) were analysed using biometabolic labelling and densitometry. Basal Hsp70/Hsc70 synthesis and percentage induction of Hsp70/Hsc70 synthesis were significantly correlated (r=−0.57, p<0.0001), and described most accurately by an exponential decay equation (R=0.68, R2=0.46). This regression equation suggests that increasing levels of basal Hsp70/Hsc70 synthesis are accompanied byan exponential decrease in the percentage induction of Hsp70/Hsc70 synthesis. The model fits data from European and non-European population groups independently, although both coefficients in the regression equation were larger for non-Europeans. This implies population group as an additional factor influencing differential HSP expression. The differential inducibility of Hsp70/Hsc70 due to variable basal synthesis of Hsp70/Hsc70 and based upon population group may contribute to differential stress tolerance or disease susceptibility. Birkhäuser Verlag Basel,, 2000 Key words. HSP nationallicence Hsp70 nationallicence Hsc70 nationallicence basal synthesis nationallicence induction nationallicence population nationallicence human monocyte nationallicence Boshoff T. Biochemistry Division, Department of Chemistry and Biochemistry, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), Fax +27 11 489 2605, e-mail: LBO@na.rau.ac.za, ZA aut Lombard F. Department of Statistics, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), ZA aut Eiselen R. Statistical Consultation Service, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), ZA aut Bornman J. J. Kynoch (Pty.) Ltd., P.O. Box 3836, Randburg 2125 (South Africa), ZA aut Bachelet M. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, 24 rue du Faubourg Saint Jacques, Paris University V, 75014 Paris (France), FR aut Polla B. S. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, 24 rue du Faubourg Saint Jacques, Paris University V, 75014 Paris (France), FR aut Bornman* L. Biochemistry Division, Department of Chemistry and Biochemistry, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), Fax +27 11 489 2605, e-mail: LBO@na.rau.ac.za, ZA aut https://doi.org/10.1007/PL00000768 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000768 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boshoff T. Biochemistry Division, Department of Chemistry and Biochemistry, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), Fax +27 11 489 2605, e-mail: LBO@na.rau.ac.za, ZA aut NATIONALLICENCE 700 1- Lombard F. Department of Statistics, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), ZA aut NATIONALLICENCE 700 1- Eiselen R. Statistical Consultation Service, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), ZA aut NATIONALLICENCE 700 1- Bornman J. J. Kynoch (Pty.) Ltd., P.O. Box 3836, Randburg 2125 (South Africa), ZA aut NATIONALLICENCE 700 1- Bachelet M. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, 24 rue du Faubourg Saint Jacques, Paris University V, 75014 Paris (France), FR aut NATIONALLICENCE 700 1- Polla B. S. Laboratoire de Physiologie Respiratoire, UFR Cochin Port-Royal, 24 rue du Faubourg Saint Jacques, Paris University V, 75014 Paris (France), FR aut NATIONALLICENCE 700 1- Bornman* L. Biochemistry Division, Department of Chemistry and Biochemistry, Rand Afrikaans University, P.O. Box 524, Auckland Park 2006 (South Africa), Fax +27 11 489 2605, e-mail: LBO@na.rau.ac.za, ZA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer