caa a22 4500 475845722 CHVBK 20180406123913.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/PL00000710 doi (NATIONALLICENCE)springer-10.1007/PL00000710 Inhibition of xanthine oxidase by liquiritigenin and isoliquiritigenin isolated from Sinofranchetia chinensis [Elektronische Daten] [L. D. Kong, Y. Zhang, X. Pan, R. X. Tan, C. H. K. Cheng*] Abstract.: The methanol extract of the stem of Sinofranchetia chinensis inhibited the activity of xanthine oxidase in vitro. Bioassay-guided purification led to the isolation of liquiritigenin and isoliquiritigenin as the main xanthine oxidase inhibitors. This inhibition of enzyme activity was found to be dose dependent, with an IC50 value of approximately 49.3 μM for liquiritigenin and 55.8 μM for isoliquiritigenin. Lineweaver-Burk transformation of the inhibition data indicated that the inhibition was of a mixed type for both liquiritigenin and isoliquiritigenin. For liquiritigenin, the Ki and KI were determined to be 14.0 μM and 151.6 μM, respectively. For isoliquiritigenin, the Ki and KI were determined to be 17.4 μM and 81.9 μM, respectively. These results suggest that these natural products could be used to treat conditions where the inhibition of xanthine oxidase is warranted. Birkhäuser Verlag Basel,, 2000 Key words.Sinofranchetia chinensis nationallicence Lardizabalaceae nationallicence liquiritigenin nationallicence isoliquiritigenin nationallicence xanthine oxidase nationallicence Kong L. D. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut Zhang Y. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut Pan X. Department of Pharmacy, Lanzhou Medical College, Lanzhou 730000 (China), CN aut Tan R. X. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut Cheng* C. H. K. Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong (China), Fax +852 2603 5123, e-mail: chkcheng@cuhk.edu.hk, CN aut https://doi.org/10.1007/PL00000710 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000710 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kong L. D. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut NATIONALLICENCE 700 1- Zhang Y. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut NATIONALLICENCE 700 1- Pan X. Department of Pharmacy, Lanzhou Medical College, Lanzhou 730000 (China), CN aut NATIONALLICENCE 700 1- Tan R. X. State Key Laboratory of Pharmaceutical Biotechnology, Institute of Pharmaceutical Biology and Phytochemistry, Nanjing University, Nanjing 210093 (China), CN aut NATIONALLICENCE 700 1- Cheng* C. H. K. Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong (China), Fax +852 2603 5123, e-mail: chkcheng@cuhk.edu.hk, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer