caa a22 4500 475845757 CHVBK 20180406123913.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/PL00000707 doi (NATIONALLICENCE)springer-10.1007/PL00000707 Heat shock effects on cell cycle progression [Elektronische Daten] [N. M. Kühl, L. Rensing*] Abstract.: In mammalian cells, short-term (acute) exposure to a moderate heat shock leads to a transient arrest of cells at mainly two cell cycle checkpoints, the G1/S and G2/M transitions. This is documented by the more or less synchronous resumption of cell cycle progression from these checkpoints during recovery. The reason for the accumulation of cells at these checkpoints may be found in activity thresholds of cyclin-dependent kinases (Cdks) at both transitions which are determined by (i) the amounts of the responsible cyclins, (ii) regulatory phosphorylation of the Cdks and (iii) the inhibition of Cdks by associated regulatory proteins (Ckis). All three regulatory systems may be subject to heat-shock-dependent changes, the amounts of Ckis, in particular, being increased. Cdk-dependent phosphorylation of the retinoblastoma protein and the subsequent release of active S-phase-specific transcription factors E2F/DP are considered as major heat-sensitive steps in cell cycle progression. Furthermore, high acute heat shock and long-term (chronic) heat treatment may lead to cell-type-specific forms of cell death. All types of responses to heat treatment are subject to adaptation after a ‘priming' treatment, probably due to higher levels of heat shock proteins. Birkhäuser Verlag Basel,, 2000 Key words. Heat shock nationallicence cell cycle nationallicence arrest nationallicence cell death nationallicence thermotolerance nationallicence cyclin-dependent protein kinases nationallicence Kühl N. M. Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, P.O. Box 330440, D-28334 Bremen (Germany), Fax +49 421 218 4042, e-mail: rensing@uni-bremen.de, DE aut Rensing* L. Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, P.O. Box 330440, D-28334 Bremen (Germany), Fax +49 421 218 4042, e-mail: rensing@uni-bremen.de, DE aut https://doi.org/10.1007/PL00000707 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000707 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kühl N. M. Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, P.O. Box 330440, D-28334 Bremen (Germany), Fax +49 421 218 4042, e-mail: rensing@uni-bremen.de, DE aut NATIONALLICENCE 700 1- Rensing* L. Institute of Cell Biology, Biochemistry and Biotechnology, University of Bremen, P.O. Box 330440, D-28334 Bremen (Germany), Fax +49 421 218 4042, e-mail: rensing@uni-bremen.de, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer