caa a22 4500 475845781 CHVBK 20180406123913.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/PL00000712 doi (NATIONALLICENCE)springer-10.1007/PL00000712 Resting potential of excitable neuroblastoma cells in weak magnetic fields [Elektronische Daten] [H. Sonnier, O. V. Kolomytkin, A. A. Marino*] Abstract.: The mechanism by which static and low-frequency magnetic fields are transduced into biological signals responsible for reported effects on brain electrical activity is not yet ascertained. To test the hypothesis that fields can cause a subthreshold change in the resting membrane potential of excitable cells, we measured changes in transmembrane current under voltage clamp produced in SH-SY5Y neuroblastoma cells, using the patch-clamp method in the whole-cell configuration. In separate experiments, cells were exposed to static fields of 1, 5, and 75 G, to time-varying fields of 1 and 5 G, and to combined static and time-varying fields tuned for resonance of Na+, K+, Ca2+, or H+. To increase sensitivity, measurements were made on cells connected by gap junctions. For each cell, the effect of the field was evaluated on the basis of 100 trials consisting of a 5-s exposure immediately followed by a 5-s control period. In each experiment, the field had no discernible effect on the transmembrane current in the vicinity of zero current (−50 mV voltage clamp). The sensitivity of the measuring system was such that we would have detected a current corresponding to a change in membrane potential as small as 38 μV. Consequently, if sensitivity of mammalian cells to magnetic fields is mediated by subthreshold changes in membrane potential, as in sensory transduction of sound, light, and other stimuli, then the ion channels responsible for the putative changes are probably present only in specialized sensory neurons or neuroepithelial cells. A change in transmembrane potential in response to magnetic fields is not a general property of excitable cells in culture. Birkhäuser Verlag Basel,, 2000 Key words. Neuron nationallicence magnetic field nationallicence transduction nationallicence membrane potential nationallicence membrane current nationallicence ion resonance nationallicence Sonnier H. Department of Orthopedic Surgery, LSU Medical Center in Shreveport, P.O. Box 33932, Shreveport (Louisiana 71130-3932, USA), Fax +1 318 675 6186, e-mail: amarino@lsumc.edu, US aut Kolomytkin O. V. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142292 (Russia), RU aut Marino* A. A. Department of Orthopedic Surgery, LSU Medical Center in Shreveport, P.O. Box 33932, Shreveport (Louisiana 71130-3932, USA), Fax +1 318 675 6186, e-mail: amarino@lsumc.edu, US aut https://doi.org/10.1007/PL00000712 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000712 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sonnier H. Department of Orthopedic Surgery, LSU Medical Center in Shreveport, P.O. Box 33932, Shreveport (Louisiana 71130-3932, USA), Fax +1 318 675 6186, e-mail: amarino@lsumc.edu, US aut NATIONALLICENCE 700 1- Kolomytkin O. V. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142292 (Russia), RU aut NATIONALLICENCE 700 1- Marino* A. A. Department of Orthopedic Surgery, LSU Medical Center in Shreveport, P.O. Box 33932, Shreveport (Louisiana 71130-3932, USA), Fax +1 318 675 6186, e-mail: amarino@lsumc.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer