caa a22 4500 475846095 CHVBK 20180406123914.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/PL00000727 doi (NATIONALLICENCE)springer-10.1007/PL00000727 Conformational dynamics of the β2-microglobulin C terminal in the cell-membrane-anchored major histocompatibility complex type I [Elektronische Daten] [M. Massa, P. Mangione, P. Pignatti, M. Stoppini, G. Zanotti, P. Arcidiaco, G. Merlini, G. Ferri, V. Bellotti*] Abstract.: We have recently described an anti-β 2-microglobulin (β2-m) monoclonal antibody (mAb 14H3) capable of recognizing the epitope 92-99 of the protein in the monomeric native state as well as in the fibrillar polymeric state, but not in the major histocompatibility complex type I (MHCI) anchored to the cell membrane. In the present study, we investigated the molecular basis for the inaccessibility of the C-terminal end of β2-m in the MHCI complex, and demonstrated that mAb 14H3 binds the soluble fraction of the MHCI complex with a Kd of 0.3 μM. An interaction between the complex and the membrane protects β2-m from immunological recognition at the MHCI level. This protection from antibody recognition can be weakened by procedures such as heat shock or γ irradiation that perturb the membrane structure and commit the cell to the apoptotic pathway. mAb 14H3 can recognize MHCI in a transient state that most likely precedes β2-m shedding and may be proposed as a useful tool for dynamic analysis of MHCI conformational modifications. Birkhäuser Verlag Basel,, 2000 Key words. Amyloidosis nationallicence major histocompatibility antigen type I nationallicence apoptosis nationallicence β2-microglobulin nationallicence monoclonal antibody nationallicence Massa M. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut Mangione P. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut Pignatti P. Department of Pediatrics, University of Pavia (Italy), IT aut Stoppini M. Department of Biochemistry, University of Pavia, via Taramelli 3b, I-27100, Pavia (Italy), Fax +39 382 423108, e-mail:vbellot@unipv.it, IT aut Zanotti G. Institute of Organic Chemistry, University of Padova (Italy), IT aut Arcidiaco P. Centro Grandi Strumenti, University of Pavia (Italy), IT aut Merlini G. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut Ferri G. Department of Biochemistry, University of Pavia, via Taramelli 3b, I-27100, Pavia (Italy), Fax +39 382 423108, e-mail:vbellot@unipv.it, IT aut Bellotti* V. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut https://doi.org/10.1007/PL00000727 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000727 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Massa M. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut NATIONALLICENCE 700 1- Mangione P. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut NATIONALLICENCE 700 1- Pignatti P. Department of Pediatrics, University of Pavia (Italy), IT aut NATIONALLICENCE 700 1- Stoppini M. Department of Biochemistry, University of Pavia, via Taramelli 3b, I-27100, Pavia (Italy), Fax +39 382 423108, e-mail:vbellot@unipv.it, IT aut NATIONALLICENCE 700 1- Zanotti G. Institute of Organic Chemistry, University of Padova (Italy), IT aut NATIONALLICENCE 700 1- Arcidiaco P. Centro Grandi Strumenti, University of Pavia (Italy), IT aut NATIONALLICENCE 700 1- Merlini G. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut NATIONALLICENCE 700 1- Ferri G. Department of Biochemistry, University of Pavia, via Taramelli 3b, I-27100, Pavia (Italy), Fax +39 382 423108, e-mail:vbellot@unipv.it, IT aut NATIONALLICENCE 700 1- Bellotti* V. Biotechnology Research Laboratory, IRCCS Policlinico S. Matteo, Pavia (Italy), IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer