caa a22 4500 475846117 CHVBK 20180406123914.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/PL00000726 doi (NATIONALLICENCE)springer-10.1007/PL00000726 The structure and function of initiation factors in eukaryotic protein synthesis [Elektronische Daten] [T. V. Pestova*, C. U. T. Hellen] Abstract.: Protein synthesis is one of the most complex cellular processes, involving numerous translation components that interact in multiple sequential steps. The most complex stage in protein synthesis is the initiation process. It involves initiation factor-mediated assembly of a 40S ribosomal subunit and initiator tRNA into a 48S initiation complex at the initiation codon of an mRNA and subsequent joining of a 60S ribosomal subunit to form a translationally active 80S ribosome. The basal set of factors required for translation initiation has been determined, and biochemical, genetic, and structural studies are now beginning to reveal details of their individual functions in this process. The mechanism of translation initiation has also been found to be influenced significantly by structural properties of the 5′ and 3′ termini of individual mRNAs. This review describes some of the major developments in elucidating molecular details of the mechanism of initiation that have occurred over the last decade. Birkhäuser Verlag Basel,, 2000 Key words. Eukaryotic initiation factor nationallicence internal ribosomal entry site nationallicence mRNA nationallicence ribosome nationallicence translation nationallicence Pestova* T. V. Department of Microbiology and Immunology, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Box 44, Brooklyn (New York 11203, USA), Fax +1 718 270-2656, e-mail: tpestova@netmail.hscbklyn.edu, US aut Hellen C. U. T. Department of Microbiology and Immunology, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Box 44, Brooklyn (New York 11203, USA), Fax +1 718 270-2656, e-mail: tpestova@netmail.hscbklyn.edu, US aut https://doi.org/10.1007/PL00000726 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000726 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pestova* T. V. Department of Microbiology and Immunology, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Box 44, Brooklyn (New York 11203, USA), Fax +1 718 270-2656, e-mail: tpestova@netmail.hscbklyn.edu, US aut NATIONALLICENCE 700 1- Hellen C. U. T. Department of Microbiology and Immunology, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, Box 44, Brooklyn (New York 11203, USA), Fax +1 718 270-2656, e-mail: tpestova@netmail.hscbklyn.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer