caa a22 4500 475846206 CHVBK 20180406123914.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s000180050045 doi (NATIONALLICENCE)springer-10.1007/s000180050045 Pycnogenol inhibits tumor necrosis factor-α-induced nuclear factor kappa B activation and adhesion molecule expression in human vascular endothelial cells [Elektronische Daten] [Q. Peng, Z. Wei, B. H. S. Lau*] Abstract.: The transcriptional regulatory protein nuclear factor kappa B (NF-κB) participates in the control of gene expression of many modulators of inflammatory and immune responses, including vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). The heightened expression of these adhesion molecules has been reported to play a critical role in atherosclerosis, inflammation, ischemic vascular disorders, diabetes, and cancer metastasis. In the present study, we investigated the effect of pycnogenol, an antioxidant phytochemical, on the activation of NF-κB and the induction of VCAM-1 and ICAM-1 in tumor necrosis factor (TNF)-α-treated human umbilical vein endothelial cells (HUVECs). Gel-shift analysis of HUVEC demonstrated that pretreatment with pycnogenol exhibited a concentration-dependent suppression of TNF-α-induced activation of NF-κB. Induction of VCAM-1 and ICAM-1 surface expression by TNF-α was dose-dependently reduced by pycnogenol. TNF-α significantly increased the release of superoxide anion and hydrogen peroxide from HUVECs. Pycnogenol dose-dependently inhibited their release. The ability of pycnogenol to inhibit NF-κB activation and VCAM-1 and ICAM-1 expression suggests that this phytochemical may play an important role in halting or preventing the atherogenic process. Birkhäuser Verlag Basel,, 2000 Key words. Pycnogenol nationallicence nuclear factor kappa B nationallicence adhesion molecule nationallicence antioxidant nationallicence human umbilical vein endothelial cell nationallicence Peng Q. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut Wei Z. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut Lau* B. H. S. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut https://doi.org/10.1007/s000180050045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050045 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Q. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut NATIONALLICENCE 700 1- Wei Z. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut NATIONALLICENCE 700 1- Lau* B. H. S. Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda (California 92350, USA), Fax +1 909 558 4035, e-mail: bLau@som.llu.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer