caa a22 4500 47584629X CHVBK 20180406123915.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s000180050044 doi (NATIONALLICENCE)springer-10.1007/s000180050044 Novel insecticidal toxins from nematode-symbiotic bacteria [Elektronische Daten] [R. H. ffrench-Constant*, D. J. Bowen] Abstract.: The current strategy of using transgenic crops expressing insecticidal protein toxins is placing increasing emphasis on the discovery of novel toxins, beyond those already derived from the bacterium Bacillus thuringiensis. Here we review the cloning of four insecticidal toxin complex (tc) encoding genes from a different bacterium Photorhabdus luminescens and of similar gene sequences from Xenorhabdus nematophilus. Both these bacteria occupy the gut of entomopathogenic nematodes and are released into the insect upon invasion by the nematode. In the insect the bacteria presumably secrete these insecticidal toxins, as well as a range of other antimicrobials, to establish the insect cadaver as a monocultural breeding ground for both bacteria and nematodes. In this review, the protein biochemistry and structure of the tc encoding loci are discussed in relation to their observed toxicity and histopathology. These toxins may prove useful as alternatives to those derived from B. thuringiensis for deployment in insect-resistant transgenic plants. Birkhäuser Verlag Basel,, 2000 Key words. Insecticidal protein toxins nationallicence Bacillus thuringiensis nationallicence Photorhabdus luminescens nationallicence Xenorhabdus nematophilus nationallicence ffrench-Constant* R. H. Department of Biology and Biochemistry, South Building, University of Bath, Bath BA2 7AY (United Kingdom), Fax +44 1225 826779, e-mail: bssrfc@bath.ac.uk, GB aut Bowen D. J. Department of Entomology, University of Wisconsin-Madison, Madison (Wisconsin 53706, USA), e-mail: djbowen@facstaff.wisc.edu, US aut https://doi.org/10.1007/s000180050044 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s000180050044 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- ffrench-Constant* R. H. Department of Biology and Biochemistry, South Building, University of Bath, Bath BA2 7AY (United Kingdom), Fax +44 1225 826779, e-mail: bssrfc@bath.ac.uk, GB aut NATIONALLICENCE 700 1- Bowen D. J. Department of Entomology, University of Wisconsin-Madison, Madison (Wisconsin 53706, USA), e-mail: djbowen@facstaff.wisc.edu, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer