caa a22 4500 475846400 CHVBK 20180406123915.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/PL00000694 doi (NATIONALLICENCE)springer-10.1007/PL00000694 Resveratrol-type oligostilbenes from Iris clarkei antagonize 20-hydroxyecdysone action in the Drosophila melanogaster BII cell line [Elektronische Daten] [K. Keckeis, S. D. Sarker**, L. Dinan*] Abstract.: Bioassay-guided high-performance liquid chromatography analysis of a MeOH extract of Iris clarkei seeds yielded the resveratrol-type oligomeric stilbenes, ampelopsin B and α-viniferin, which antagonize the action of 20-hydroxyecdysone; with a 20-hydroxyecdysone concentration of 50 nM, the ED50 values were 33 μM and 10 μM, respectively. The structures of these compounds were determined by spectroscopic analysis, notably ultraviolet, liquid secondary ion mass spectrometry and modern one- and two-dimensional nuclear magnetic resonance techniques. Birkhäuser Verlag Basel,, 2000 Key words. Antagonist nationallicence resveratrol nationallicence stilbene nationallicence ecdysteroid nationallicence Iris clarkei Iridaceae nationallicence receptor nationallicence steroid hormone nationallicence Keckeis K. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut Sarker** S. D. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut Dinan* L. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut https://doi.org/10.1007/PL00000694 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00000694 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keckeis K. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut NATIONALLICENCE 700 1- Sarker** S. D. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut NATIONALLICENCE 700 1- Dinan* L. Department of Biological Sciences, University of Exeter, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS (UK), e-mail: l.n.dinan@exeter.ac.uk, UA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer