caa a22 4500 475848128 CHVBK 20180406123920.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00007509 doi (NATIONALLICENCE)springer-10.1007/PL00007509 Effect of cisplatin on the expression of glutathione- S -transferase in the cochlea of the rat [Elektronische Daten] [John S. Touliatos, Leslie Neitzel, Craig Whitworth, Leonard P. Rybak, Mokenge Malafa] Glutathione-S-transferase (GST) has been found to conjugate glutathione (GSH) to diverse electrophiles and play a major role in the detoxification of alkylating and platinating agents. However, there is little information regarding the pattern of GST expression in the cochlea. Although cisplatin is ototoxic, its effect on GST in the cochlea is unknown. In the present study we investigated the pattern of GST expression in control and cisplatin-treated cochleas by using the laboratory rat as animal model. Sixteen mature rats were randomly assigned to control or cisplatin groups. After treatment, cochleas were procured and tissues stained by immunohistochemical methods to detect the presence of GST. Optical density measurements were determined to gauge intensity of GST immunostaining. Strong positive GST immunostaining was demonstrated in all cochleas, with the most intense staining found in the spiral ligament and the least in Reissner's membrane. Mean optic density scores were lower for cisplatin-treated cochleas than for control cochleas in all areas analyzed. These findings showed that GST is expressed throughout the rat cochlea, with cisplatin treatment causing its decreased expression. Springer-Verlag Berlin Heidelberg, 2000 Key words Cochlea nationallicence Cisplatin nationallicence Glutathione-S-transferases-Ototoxicity nationallicence Touliatos John S. Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut Neitzel Leslie Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut Whitworth Craig Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut Rybak Leonard P. Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut Malafa Mokenge Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut https://doi.org/10.1007/PL00007509 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00007509 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Touliatos John S. Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut NATIONALLICENCE 700 1- Neitzel Leslie Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut NATIONALLICENCE 700 1- Whitworth Craig Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut NATIONALLICENCE 700 1- Rybak Leonard P. Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut NATIONALLICENCE 700 1- Malafa Mokenge Department of Surgery, Southern Illinois University, School of Medicine, P.O. Box 19638, Springfield, IL 62794-9638, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer