caa a22 4500 475848195 CHVBK 20180406123920.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004059900220 doi (NATIONALLICENCE)springer-10.1007/s004059900220 Altered genotoxicity in mucosal cells of head and neck cancer patients due to environmental pollutants [Elektronische Daten] [N. H. Kleinsasser, Herbert Weissacher, Ernst R. Kastenbauer, Peter Dirschedl, Barbara C. Wallner, Ulrich A. Harréus] The complexity of carcinogenesis in squamous cell cancer (SCC) of the upper aerodigestive tract requires examining environmental risk factors, including mutagen sensitivities to xenobiotics. Three environmental, occupational, and habitual pollutants - dibutylphthalate (DBP), diisobutylphthalate (DiBP), and N′nitrosodiethylamine (NDELA) - were submitted to genotoxicity testing on mucosal biopsy specimens of tumor and nontumor patients in vitro. The single-cell microgel electrophoresis (Comet) assay was applied to detect DNA strand breaks in human epithelial cells of the pharynx and larynx from nontumor patients, patients with SCC of the oropharynx and patients with SCC of the larynx. Genotoxicity was found for DBP, DiBP, and NDELA in cells derived from nontumor and tumor patients. With respect to phthalates, Olive tail moment (OTM) levels were higher in patients with SCC of the oropharynx and SCC of the larynx (P < 0.01), the latter showing even more pronounced genotoxicity for DiBP. Testing epithelial cells of the patients with either oropharyngeal or laryngeal SCC for NDELA demonstrated results similar to the nontumor patients. Present findings indicate heterogeneous mutagen sensitivities to some but not all xenobiotics. Springer-Verlag Berlin Heidelberg, 2000 Keywords Genotoxicity nationallicence Carcinogenesis nationallicence Aerodigestive squamous cell carcinoma nationallicence Phthalates nationallicence Nitrosamines nationallicence Kleinsasser N. H. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut Weissacher Herbert Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut Kastenbauer Ernst R. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut Dirschedl Peter Department of Biometrie and Epidemiology, Ludwig Maximilians University of Munich, Germany, DE aut Wallner Barbara C. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut Harréus Ulrich A. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut https://doi.org/10.1007/s004059900220 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004059900220 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kleinsasser N. H. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut NATIONALLICENCE 700 1- Weissacher Herbert Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut NATIONALLICENCE 700 1- Kastenbauer Ernst R. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut NATIONALLICENCE 700 1- Dirschedl Peter Department of Biometrie and Epidemiology, Ludwig Maximilians University of Munich, Germany, DE aut NATIONALLICENCE 700 1- Wallner Barbara C. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut NATIONALLICENCE 700 1- Harréus Ulrich A. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University of Munich, Pettenkoferstrasse 4a, 80336 Munich, Germany e-mail: norbert.kleinsasser@hno.med.uni-muenchen.de, Tel.: +49-89-51603982, Fax: +49-89-51603919, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer