caa a22 4500 47584825X CHVBK 20180406123921.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s004059900218 doi (NATIONALLICENCE)springer-10.1007/s004059900218 Cytokines in children with otitis media with effusion [Elektronische Daten] [B. Skotnicka, Elżbieta Hassmann] We assayed 38 middle ear effusions from 23 children aged 4-13 years (mean 7) undergoing tympanostomy tube placements. All fluid was assayed for tumor necrosis factor (TNF) α, interleukin (IL) 1β, IL-8, and IL-10. Cytokine concentrations were measured by means of an enzyme-linked immunosorbent assay. Detectable levels of IL-1β, IL-8, and IL-10 were found in all of the effusions. TNF-α was detected in 18 of the middle ear effusions (47.4%). The mean concentration of TNF-α, IL-1β, IL-8, and IL-10 was, respectively, 0.423 ± 1.39, 30.58 ± 68.7, 7001.9 ± 6743, and 56 ± 58.7 pg/ml. There was a strong, statistically significant correlation between the concentrations of TNF-α and IL-1β (r = 0.87, P = 0.001) and between IL-1β and IL-8 (r = 0.53, P = 0.001). There was no correlation between the concentrations of IL-10 and other cytokines examined or between tympanic membrane pathology and the concentrations of TNF-α, IL-1β, IL-8, or IL-10. The presence of IL-10 in middle ear effusions may be one of the causes of a lack of clinical features of acute inflammation and may lead to a chronic inflammatory state. Springer-Verlag Berlin Heidelberg, 2000 Keywords Otitis media with effusion nationallicence Cytokines nationallicence Enzyme-linked immunosorbent assay nationallicence Skotnicka B. Department of Pediatric Otorhinolaryngology, University Children's Hospital, Waszyngtona 17, 15-274 Białystok, Poland, PL aut Hassmann Elżbieta Department of Pediatric Otorhinolaryngology, University Children's Hospital, Waszyngtona 17, 15-274 Białystok, Poland, PL aut https://doi.org/10.1007/s004059900218 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004059900218 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skotnicka B. Department of Pediatric Otorhinolaryngology, University Children's Hospital, Waszyngtona 17, 15-274 Białystok, Poland, PL aut NATIONALLICENCE 700 1- Hassmann Elżbieta Department of Pediatric Otorhinolaryngology, University Children's Hospital, Waszyngtona 17, 15-274 Białystok, Poland, PL aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer