caa a22 4500 475848292 CHVBK 20180406123921.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004050000280 doi (NATIONALLICENCE)springer-10.1007/s004050000280 Cochlear microinjection and its effects upon auditory function in the guinea pig [Elektronische Daten] [S. T. Kho, R. M. Pettis, A. N. Mhatre, A. K. Lalwani] Microinjection through the round window membrane has been found to represent a method for vector delivery in intracochlear gene transfer in animal models but breaches the round window membrane, making it necessary to evaluate animals for possible postinjection hearing loss. In the present study healthy guinea pigs were evaluated for their baseline click auditory brainstem response (ABR) thresholds. Each animal was then injected with saline via the round window membrane. After 1 week auditory function was evaluated by click ABR. Animals with increased ABR thresholds were retested at 4 weeks. Animals with 1-week postoperative ABRs similar to baseline were not retested. Results showed that postoperative ABR thresholds in five animals (71%) remained unchanged from baseline, while two animals had increases of 20-¶25 dB in ABRs after 1 week but recovered baseline ABRs after 4 weeks. The mean baseline ABR threshold was ¶25.7 dB and was 27.9 dB after 1 week after injection. The difference between preoperative and 1-week postoperative averages was not significant (P = 0.707). In this preliminary study saline microinjection through the round window membrane did not cause permanent hearing loss in the guinea pigs tested, and any damage caused by microinjection appeared to be reversible. Springer-Verlag Berlin Heidelberg, 2000 Key words Intracochlear gene therapy nationallicence Auditory ¶brainstem response thresholds nationallicence Round window ¶microinjection nationallicence Kho S. T. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut Pettis R. M. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut Mhatre A. N. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut Lalwani A. K. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut https://doi.org/10.1007/s004050000280 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050000280 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kho S. T. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut NATIONALLICENCE 700 1- Pettis R. M. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut NATIONALLICENCE 700 1- Mhatre A. N. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut NATIONALLICENCE 700 1- Lalwani A. K. Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology - Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue U490A, San Francisco, CA 94117, USA e-mail: lalwani@itsa.ucsf.edu, Tel.: +1-415-5024880, Fax: +1-415-4762169, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer