caa a22 4500 475848578 CHVBK 20180406123921.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004050050212 doi (NATIONALLICENCE)springer-10.1007/s004050050212 Analysis of aromatic DNA adducts in laryngeal biopsies [Elektronische Daten] [J. Banaszewski, Z. Szmeja, W. Szyfter, K. Szyfter, P. Baranczewski, L. Möller] Epidemiological studies have confirmed the correlation between tobacco smoking, environmental pollution and the incidence of cancers of the respiratory tract. The occurrence of laryngeal cancer in Poland is relatively high compared to other European countries. Since 1969 the mortality related to larynx cancer appears to be increasing. Tobacco smoke contains an abundance of such carcinogenic compounds as polycyclic aromatic hydrocarbons (PAH), aromatic amines and N-nitrosoamines, which can react with DNA and form adducts. We analyzed aromatic DNA adducts in laryngeal tissues from patients with primary laryngeal, which was confirmed histopathologically to be squamous cell carcinoma. The group consisted of 33 patients (5 women and 28 men). Total laryngectomy was performed in patients. A detergent-phenol method was used for DNA isolation. Aromatic DNA adducts were analyzed by a 32P-postlabelling technique with butanol extraction and high performance liquid chromatography. The presence of aromatic DNA adducts was demonstrated in all tissues. Large interindividual differences of DNA adduct levels were seen in each tissue studied. There was a higher mean level of DNA adducts in interarytenoid area non-tumors (51.96/108± 91.71 NN) than in non-tumor tissue elswhere (46.91/108± 46.36 NN) and tumor tissue (43.52/108± 45.88 NN). Adduct levels were correlated with age, sex, cigarette smoking and TNM stage. Springer-Verlag Berlin Heidelberg, 2000 Key words Laryngeal cancer nationallicence Aromatic DNA adducts nationallicence Postlabelling nationallicence Tissues nationallicence Banaszewski J. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut Szmeja Z. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut Szyfter W. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut Szyfter K. Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland, PL aut Baranczewski P. Department of Bioscience, Center for Nutrition and Toxicology, Unit for Analytical Toxicology, Karolinska Institutet, Stockholm, Sweden, SE aut Möller L. Department of Bioscience, Center for Nutrition and Toxicology, Unit for Analytical Toxicology, Karolinska Institutet, Stockholm, Sweden, SE aut https://doi.org/10.1007/s004050050212 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050050212 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Banaszewski J. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut NATIONALLICENCE 700 1- Szmeja Z. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut NATIONALLICENCE 700 1- Szyfter W. Department of Otolaryngology, University School of Medicine, ul. Przybyszewskiego 49, 60-355 Poznań, Poland e-mail: jbanasz@eucalyptus.usoms.poznan.pl, Tel.: +48 61 869 13 87, Fax: +48 61 869 16 90, PL aut NATIONALLICENCE 700 1- Szyfter K. Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland, PL aut NATIONALLICENCE 700 1- Baranczewski P. Department of Bioscience, Center for Nutrition and Toxicology, Unit for Analytical Toxicology, Karolinska Institutet, Stockholm, Sweden, SE aut NATIONALLICENCE 700 1- Möller L. Department of Bioscience, Center for Nutrition and Toxicology, Unit for Analytical Toxicology, Karolinska Institutet, Stockholm, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer