caa a22 4500 475848691 CHVBK 20180406123922.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s004050000285 doi (NATIONALLICENCE)springer-10.1007/s004050000285 Lipopolysaccharide-induced nasal cytokine response: a dose-response evaluation [Elektronische Daten] [B. Danuser, Hanni Rebsamen, Christoph Weber, Helmut Krueger] Information on the dose-response relationship is a prerequisite to defining the non-response threshold of exposure. We investigated whether nasal lipopolysaccharide (LPS) challenges induce an inflammatory response in a dose-dependent way. In three settings nasal lavage was performed before, and 20 min, 1, 6, 23, and 29 h after instillation of 0 μg, 10 μg, and 40 μg LPS for 10 s, in seven healthy subjects. Lavage fluids were analysed for concentrations of interleukin-6 (IL-6), IL-8, tumour necrosis factor-α (TNF-α), histamine, and albumin. Symptoms were recorded by questionnaire and spirometric lung function was assessed after each lavage. The instillation of 40 μg LPS caused a small increase in nasal symptoms. TNF-α was below the detection limit (0.5 pg/ml) in most subjects and, like IL-8 and albumin, showed no relation to the LPS challenge. IL-6 increased over twofold with 10 μg LPS and over 13-fold with 40 μg LPS, with a peak at 6 h after LPS provocation, and the repeated design ANOVA was significant for dose and for time. Six hours after the 40 μg LPS challenge the histamine level significantly increased compared to the saline treatment. We conclude that short-lasting instillation of LPS causes a dose-dependent IL-6 release in the upper airways and minor nasal symptoms. Springer-Verlag Berlin Heidelberg, 2000 Keywords Lipopolysaccharides nationallicence Nasal lavage nationallicence Cytokines nationallicence Dose response nationallicence Nasal symptoms nationallicence Histamine nationallicence Danuser B. Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut Rebsamen Hanni Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut Weber Christoph Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut Krueger Helmut Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut https://doi.org/10.1007/s004050000285 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050000285 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Danuser B. Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut NATIONALLICENCE 700 1- Rebsamen Hanni Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut NATIONALLICENCE 700 1- Weber Christoph Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut NATIONALLICENCE 700 1- Krueger Helmut Institut für Hygiene und Arbeitsphysiolgie, Swiss Federal Institute of Technology, Clausiusstrasse 25, 8092 Zurich, Switzerland e-mail: danuser@iha.bepr.ethz.ch, Tel.: +41-1-6323986, Fax: +41-1-6321287, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer