caa a22 4500 475848845 CHVBK 20180406123922.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004050050222 doi (NATIONALLICENCE)springer-10.1007/s004050050222 Effects of macrolides on interleukin-8 secretion from human nasal epithelial cells [Elektronische Daten] [K. Fujita, Takeshi Shimizu, Yuich Majima, Yasuo Sakakura] Low-dose, long-term macrolide treatment has recently been reported to be very effective in patients with chronic airway diseases. We examined the in vivo and in vitro effects of 14-membered macrolide antibiotics erythromycin (EM) and clarithromycin (CAM) on interleukin (IL)-8 secretion from human nasal epithelial cells. Fifteen patients with chronic sinusitis received macrolide treatment (CAM 400 mg/day) for 1 to 3 months. The number of infiltrated neutrophils and IL-8 concentrations in the nasal discharges of these patients decreased significantly at 1 to 2 months after the treatment. In vitro effects of EM and CAM on IL-8 secretion were examined in nasal epithelial cells cultured at the air-liquid interface. After 14-day culture in the air-liquid interface, macrolide antibiotics were added in medium for 24 h. EM and CAM at concentrations of 10-4 M did not affect spontaneous secretions or IL-1β-induced secretions of IL-8 either apically or basolaterally. When cells were preincubated with 10-4 M CAM for 7 days, the IL-1β-induced secretion of IL-8 decreased significantly. However, no difference was observed between the effects of 10-4 M CAM and 10-4 M josamycin, a 16-membered macrolide. These results suggest that macrolide treatment inhibits neutrophil infiltration and IL-8 secretion in nasal epithelium in vivo and that these clinical effects depend on a mechanism other than the direct action of macrolide on nasal epithelial cells. Springer-Verlag Berlin Heidelberg, 2000 Key words Chronic sinusitis nationallicence Interleukin-8 nationallicence Human nasal epithelial cells nationallicence Macrolide antibiotics nationallicence Fujita K. Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut Shimizu Takeshi Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut Majima Yuich Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut Sakakura Yasuo Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut https://doi.org/10.1007/s004050050222 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050050222 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujita K. Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut NATIONALLICENCE 700 1- Shimizu Takeshi Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut NATIONALLICENCE 700 1- Majima Yuich Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut NATIONALLICENCE 700 1- Sakakura Yasuo Department of Otorhinolaryngology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, Japan Tel.: ++81-59-232-1111, ext. 5637, Fax: ++81-59-231-5218, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer