caa a22 4500 47584890X CHVBK 20180406123922.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004050050219 doi (NATIONALLICENCE)springer-10.1007/s004050050219 A new model for investigating hair cell degeneration in the guinea pig following damage of the stria vascularis using a photochemical reaction [Elektronische Daten] [Shuji Ocho, Satoshi Iwasaki, Kazuo Umemura, Tomoyuki Hoshino] We have established a new model for investigating the relationship between cochlear lateral wall damage and sensory cell degeneration in guinea pigs by using a photochemical reaction between the systemic injection of Rose Bengal (RB) and controlled green light irradiation to the cochlea. The photochemical reaction produced a reactive oxygen species, which then damaged the endothelium. This triggered platelet adhesion and aggregation at the site of endothelial injury to produce thrombi and affect microcirculation in the lateral wall at the site of irradiation. Changes were studied under a scanning electron microscope (SEM), and compound action potentials (CAP) were measured. SEM observations after tangential illumination of the cochlear wall revealed degeneration of the stria vascuralis (SV). Specific morphological findings at 24 h included delayed degeneration of the outer hair cells concurrent with a significant increase in the CAP. Based on these findings, we suggest that degeneration of the SV was a direct result of the photochemical reaction, but CAP changes and sensory hair cell damage were secondarily caused by SV degeneration. Springer-Verlag Berlin Heidelberg, 2000 Key words Hearing loss nationallicence Photochemical reaction nationallicence Cochlear compound action potentials nationallicence Scanning electron microscopy nationallicence Strial degeneration nationallicence Ocho Shuji Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan, JP aut Iwasaki Satoshi Department of Otolaryngology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan e-mail: ocho@hama-med.ac.jp, Tel.: +81 53 435 2252, Fax: +81 53 435 2253, JP aut Umemura Kazuo Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan, JP aut Hoshino Tomoyuki Department of Otolaryngology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan e-mail: ocho@hama-med.ac.jp, Tel.: +81 53 435 2252, Fax: +81 53 435 2253, JP aut https://doi.org/10.1007/s004050050219 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050050219 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ocho Shuji Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan, JP aut NATIONALLICENCE 700 1- Iwasaki Satoshi Department of Otolaryngology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan e-mail: ocho@hama-med.ac.jp, Tel.: +81 53 435 2252, Fax: +81 53 435 2253, JP aut NATIONALLICENCE 700 1- Umemura Kazuo Department of Pharmacology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan, JP aut NATIONALLICENCE 700 1- Hoshino Tomoyuki Department of Otolaryngology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu, 431-3192, Japan e-mail: ocho@hama-med.ac.jp, Tel.: +81 53 435 2252, Fax: +81 53 435 2253, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer