caa a22 4500 475849043 CHVBK 20180406123923.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004050000261 doi (NATIONALLICENCE)springer-10.1007/s004050000261 Possible roles of nitric oxide in the physiology and pathophysiology of the guinea pig cochlea [Elektronische Daten] [K. Gosepath, U. R. Heinrich, U. Ecke, J. Maurer, R. Amedee, W. J. Mann] Two nitric oxide synthase isoforms (NOS-I and NOS-III) are present in the mammalian cochlea, and many regulatory functions of the inner ear could be mediated by NO. In this study, cochlear potentials and immunohistochemical measures were analyzed by changing the concentration of NO in the guinea pig during intracochlear and intravenous application of an NOS inhibitor and an NO donor. In anesthetized guinea pigs the NOS inhibitor N G-nitro-L-arginine-methylester (L-NAME) was applied by intracochlear perfusion or intravenously. The cochlear potentials and blood pressure were measured during and after the application. Cochleae were removed and prepared for morphological and immunohistochemical examinations by light and electron microscopy. The same series of experiments was performed with the NO donor S-nitroso-N-acetyl-penicillamine (SNAP). Cochlear potentials were not altered during intracochlear perfusion or intravenous application of L-NAME or SNAP. With intravenous application of the substances, significant alterations in the systemic blood pressure were seen. No morphological alterations were noted but in the immunohistochemical investigations significant changes were detected by electron microscopy. Several possible functions of NO in the inner and outer hair cells, stria vascularis and ganglion cells are discussed. Our results suggest that NO has a major effect on the physiology of the inner and outer hair cells. Springer-Verlag Berlin Heidelberg, 2000 Key words NO donor nationallicence NOS inhibitor nationallicence Intracochlear perfusion nationallicence Intravenous application nationallicence Cochlear potentials nationallicence Immunohistochemical alterations nationallicence Gosepath K. HNO-Universitätsklinik, Langenbeckstrasse 1, 55101 Mainz, Germany Tel.: +49-6131177361, Fax: +49-6131176637, DE aut Heinrich U. R. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut Ecke U. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut Maurer J. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut Amedee R. Department of Otolaryngology-Head and Neck Surgery, Tulane University School of Medicine, New Orleans, LA 70112, USA, US aut Mann W. J. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut https://doi.org/10.1007/s004050000261 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050000261 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gosepath K. HNO-Universitätsklinik, Langenbeckstrasse 1, 55101 Mainz, Germany Tel.: +49-6131177361, Fax: +49-6131176637, DE aut NATIONALLICENCE 700 1- Heinrich U. R. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut NATIONALLICENCE 700 1- Ecke U. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut NATIONALLICENCE 700 1- Maurer J. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut NATIONALLICENCE 700 1- Amedee R. Department of Otolaryngology-Head and Neck Surgery, Tulane University School of Medicine, New Orleans, LA 70112, USA, US aut NATIONALLICENCE 700 1- Mann W. J. Department of Otolaryngology - Head and Neck Surgery, Johannes Gutenberg University Medical School, 55101 Mainz, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer