caa a22 4500 475849116 CHVBK 20180406123923.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004050050231 doi (NATIONALLICENCE)springer-10.1007/s004050050231 Immunolocalization of inducible nitric oxide synthase and 3-nitrotyrosine in the nasal mucosa of patients with rhinitis [Elektronische Daten] [B. -H. Kang, S. -S. Chen, L. -S. Jou, P. -K. Weng, H. -W. Wang] Since nitric oxide (NO) can be involved in multiple physiological and pathological functions, we evaluated its possible involvement and that of peroxynitrite in the pathogenesis of rhinitis. Inferior nasal turbinates were obtained from allergic rhinitis and nonallergic rhinitis patients during corrective nasal surgery. The expressions of the inducible form of nitric oxide synthase (iNOS) and the production of peroxynitrite and its metabolite 3-nitrotyrosine were examined by immunohistochemistry in consecutive tissue sections. Each section (or tissue compartment) was given a score of 0-4 according to the labeling intensity seen, with the highest number representing the highest labeling intensity. The results showed that iNOS expression was present mainly in the mucosal epithelium, vascular endothelium, and submucosal glands. A significant difference was only observed in the labeling scores of glandular tissues of the allergic group, which had a higher iNOS labeling score. We also found that sections with a higher iNOS level did not necessarily exhibit a higher 3-nitrotyrosine labeling intensity. These data suggest that iNOS-derived NO may have a role in the pathophysiology of rhinitis, especially the glandular function of allergic nasal mucosa. Moreover, our findings suggest that the production of peroxynitrite in rhinitis patients is not dependent on the level of iNOS alone. Springer-Verlag Berlin Heidelberg, 2000 Key words Rhinitis nationallicence Tissue nitric oxide nationallicence Peroxynitrite metabolism nationallicence Inducible nitric oxide synthase nationallicence Kang B. -H Institute of Undersea and Hyperbaric Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China, CN aut Chen S. -S Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut Jou L. -S Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut Weng P. -K Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut Wang H. -W Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut https://doi.org/10.1007/s004050050231 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004050050231 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kang B. -H Institute of Undersea and Hyperbaric Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China, CN aut NATIONALLICENCE 700 1- Chen S. -S Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut NATIONALLICENCE 700 1- Jou L. -S Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut NATIONALLICENCE 700 1- Weng P. -K Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut NATIONALLICENCE 700 1- Wang H. -W Department of Otolaryngology, Tri-Service General Hospital, 8 Section 3, Ting-Chow Rd, Taipei, Taiwan 100, Republic of China Tel.: +886-2-23681502, Fax: +886-2-23680014, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer